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CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling
As RNA-binding proteins, cytoplasmic polyadenylation element binding proteins (CPEBs) have drawn increasing attention for their function of controlling gene expression related to malignant transformation via post-transcriptional regulation. However, the contribution of CPEB3 to malignant development...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695424/ https://www.ncbi.nlm.nih.gov/pubmed/33146632 http://dx.doi.org/10.18632/aging.103893 |
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author | Fang, Yuxin Zhong, Qian Wang, Yadong Gu, Chuncai Liu, Side Li, Aimin Yan, Qun |
author_facet | Fang, Yuxin Zhong, Qian Wang, Yadong Gu, Chuncai Liu, Side Li, Aimin Yan, Qun |
author_sort | Fang, Yuxin |
collection | PubMed |
description | As RNA-binding proteins, cytoplasmic polyadenylation element binding proteins (CPEBs) have drawn increasing attention for their function of controlling gene expression related to malignant transformation via post-transcriptional regulation. However, the contribution of CPEB3 to malignant development in cancers is poorly understood. In this study, we explored the clinical, biological, and mechanical role of CPEB3 in colorectal cancer progression. We showed that colorectal cancer tissues exhibited dampened CPEB3 expression which was closely associated with poor prognosis in patients with colorectal cancer (47 vs. 62 months, P = 0.035, n=99). Down-regulation CPEB3 promoted proliferation, migration, and invasion in colorectal cancer cells and vice versa. Mechanistically, CPEB3 performed as an RNA binding protein binding to 3ʹUTR of JAK1 mRNA to inhibit JAK/STAT pathways in colorectal cancer cells. Knockdown of CPEB3 induced active JAK-STAT signaling, thereby triggering the proliferation and metastasis capacity of colorectal cancer cells. These results suggest that CPEB3 functions as a tumor suppressor in colorectal cancer through its post-transcriptional regulation of JAK/STAT signaling. Implications: This study identified a novel role of the RNA binding protein CPEB3 in inhibiting cell proliferation and migration as well as the underlining mechanisms in colorectal cancer cells. |
format | Online Article Text |
id | pubmed-7695424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-76954242020-12-04 CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling Fang, Yuxin Zhong, Qian Wang, Yadong Gu, Chuncai Liu, Side Li, Aimin Yan, Qun Aging (Albany NY) Research Paper As RNA-binding proteins, cytoplasmic polyadenylation element binding proteins (CPEBs) have drawn increasing attention for their function of controlling gene expression related to malignant transformation via post-transcriptional regulation. However, the contribution of CPEB3 to malignant development in cancers is poorly understood. In this study, we explored the clinical, biological, and mechanical role of CPEB3 in colorectal cancer progression. We showed that colorectal cancer tissues exhibited dampened CPEB3 expression which was closely associated with poor prognosis in patients with colorectal cancer (47 vs. 62 months, P = 0.035, n=99). Down-regulation CPEB3 promoted proliferation, migration, and invasion in colorectal cancer cells and vice versa. Mechanistically, CPEB3 performed as an RNA binding protein binding to 3ʹUTR of JAK1 mRNA to inhibit JAK/STAT pathways in colorectal cancer cells. Knockdown of CPEB3 induced active JAK-STAT signaling, thereby triggering the proliferation and metastasis capacity of colorectal cancer cells. These results suggest that CPEB3 functions as a tumor suppressor in colorectal cancer through its post-transcriptional regulation of JAK/STAT signaling. Implications: This study identified a novel role of the RNA binding protein CPEB3 in inhibiting cell proliferation and migration as well as the underlining mechanisms in colorectal cancer cells. Impact Journals 2020-11-03 /pmc/articles/PMC7695424/ /pubmed/33146632 http://dx.doi.org/10.18632/aging.103893 Text en Copyright: © 2020 Fang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Fang, Yuxin Zhong, Qian Wang, Yadong Gu, Chuncai Liu, Side Li, Aimin Yan, Qun CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling |
title | CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling |
title_full | CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling |
title_fullStr | CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling |
title_full_unstemmed | CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling |
title_short | CPEB3 functions as a tumor suppressor in colorectal cancer via JAK/STAT signaling |
title_sort | cpeb3 functions as a tumor suppressor in colorectal cancer via jak/stat signaling |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695424/ https://www.ncbi.nlm.nih.gov/pubmed/33146632 http://dx.doi.org/10.18632/aging.103893 |
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