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Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice
Plant spermatogenesis is a complex process that directly affects crop breeding. A rapid change in gene abundance occurs at early meiosis prophase, when gene regulation is selective. However, how these genes are regulated remains unknown. Here, we show that rice reproductive phasiRNAs are essential f...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695705/ https://www.ncbi.nlm.nih.gov/pubmed/33247135 http://dx.doi.org/10.1038/s41467-020-19922-3 |
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author | Zhang, Yu-Chan Lei, Meng-Qi Zhou, Yan-Fei Yang, Yu-Wei Lian, Jian-Ping Yu, Yang Feng, Yan-Zhao Zhou, Ke-Ren He, Rui-Rui He, Huang Zhang, Zhi Yang, Jian-Hua Chen, Yue-Qin |
author_facet | Zhang, Yu-Chan Lei, Meng-Qi Zhou, Yan-Fei Yang, Yu-Wei Lian, Jian-Ping Yu, Yang Feng, Yan-Zhao Zhou, Ke-Ren He, Rui-Rui He, Huang Zhang, Zhi Yang, Jian-Hua Chen, Yue-Qin |
author_sort | Zhang, Yu-Chan |
collection | PubMed |
description | Plant spermatogenesis is a complex process that directly affects crop breeding. A rapid change in gene abundance occurs at early meiosis prophase, when gene regulation is selective. However, how these genes are regulated remains unknown. Here, we show that rice reproductive phasiRNAs are essential for the elimination of a specific set of RNAs during meiotic prophase I. These phasiRNAs cleave target mRNAs in a regulatory manner such that one phasiRNA can target more than one gene, and/or a single gene can be targeted by more than one phasiRNA to efficiently silence target genes. Our investigation of phasiRNA-knockdown and PHAS-edited transgenic plants demonstrates that phasiRNAs and their nucleotide variations are required for meiosis progression and fertility. This study highlights the importance of reproductive phasiRNAs for the reprogramming of gene expression during meiotic progression and establishes a basis for future studies on the roles of phasiRNAs with a goal of crop improvement. |
format | Online Article Text |
id | pubmed-7695705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76957052020-12-03 Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice Zhang, Yu-Chan Lei, Meng-Qi Zhou, Yan-Fei Yang, Yu-Wei Lian, Jian-Ping Yu, Yang Feng, Yan-Zhao Zhou, Ke-Ren He, Rui-Rui He, Huang Zhang, Zhi Yang, Jian-Hua Chen, Yue-Qin Nat Commun Article Plant spermatogenesis is a complex process that directly affects crop breeding. A rapid change in gene abundance occurs at early meiosis prophase, when gene regulation is selective. However, how these genes are regulated remains unknown. Here, we show that rice reproductive phasiRNAs are essential for the elimination of a specific set of RNAs during meiotic prophase I. These phasiRNAs cleave target mRNAs in a regulatory manner such that one phasiRNA can target more than one gene, and/or a single gene can be targeted by more than one phasiRNA to efficiently silence target genes. Our investigation of phasiRNA-knockdown and PHAS-edited transgenic plants demonstrates that phasiRNAs and their nucleotide variations are required for meiosis progression and fertility. This study highlights the importance of reproductive phasiRNAs for the reprogramming of gene expression during meiotic progression and establishes a basis for future studies on the roles of phasiRNAs with a goal of crop improvement. Nature Publishing Group UK 2020-11-27 /pmc/articles/PMC7695705/ /pubmed/33247135 http://dx.doi.org/10.1038/s41467-020-19922-3 Text en © The Author(s) 2020, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Yu-Chan Lei, Meng-Qi Zhou, Yan-Fei Yang, Yu-Wei Lian, Jian-Ping Yu, Yang Feng, Yan-Zhao Zhou, Ke-Ren He, Rui-Rui He, Huang Zhang, Zhi Yang, Jian-Hua Chen, Yue-Qin Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice |
title | Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice |
title_full | Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice |
title_fullStr | Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice |
title_full_unstemmed | Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice |
title_short | Reproductive phasiRNAs regulate reprogramming of gene expression and meiotic progression in rice |
title_sort | reproductive phasirnas regulate reprogramming of gene expression and meiotic progression in rice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695705/ https://www.ncbi.nlm.nih.gov/pubmed/33247135 http://dx.doi.org/10.1038/s41467-020-19922-3 |
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