Cargando…

Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis

Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown...

Descripción completa

Detalles Bibliográficos
Autores principales: Jimenez, Hugo R., Edwards, Joan E., Sanderson, Ruth, Kingston-Smith, Alison H., McEwan, Neil R., Theodorou, Michael K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696013/
https://www.ncbi.nlm.nih.gov/pubmed/33187375
http://dx.doi.org/10.3390/microorganisms8111774
_version_ 1783615311222669312
author Jimenez, Hugo R.
Edwards, Joan E.
Sanderson, Ruth
Kingston-Smith, Alison H.
McEwan, Neil R.
Theodorou, Michael K.
author_facet Jimenez, Hugo R.
Edwards, Joan E.
Sanderson, Ruth
Kingston-Smith, Alison H.
McEwan, Neil R.
Theodorou, Michael K.
author_sort Jimenez, Hugo R.
collection PubMed
description Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown to affect the ability of anaerobic fungi to break down plant biomass. However, methods to reduce the non-productive lag time in batch cultures and the effect of leaf-blade cut-length and condition on the fungal fermentation are not known. Therefore, experimentation using a novel gas production approach with pre-grown, axenic cultures of Neocallimastix frontalis was performed using both fresh and air-dried perennial ryegrass leaf-blades of different cut-lengths. The methodology adopted removed the lag-phase and demonstrated the digestion of un-autoclaved leaf-blades. Fermentation of leaf-blades of 4.0 cm cut-length produced 18.4% more gas yet retained 11.2% more apparent DM relative to 0.5 cm cut-length leaf-blades. Drying did not affect fermentation by N. frontalis, although an interaction between drying and leaf-blade cut-length was noted. Removal of the lag phase and the use of un-autoclaved substrates are important when considering the biotechnological potential of anaerobic fungi. A hypothesis based upon sporulation at cut surfaces is proposed to describe the experimental results.
format Online
Article
Text
id pubmed-7696013
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76960132020-11-29 Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis Jimenez, Hugo R. Edwards, Joan E. Sanderson, Ruth Kingston-Smith, Alison H. McEwan, Neil R. Theodorou, Michael K. Microorganisms Article Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown to affect the ability of anaerobic fungi to break down plant biomass. However, methods to reduce the non-productive lag time in batch cultures and the effect of leaf-blade cut-length and condition on the fungal fermentation are not known. Therefore, experimentation using a novel gas production approach with pre-grown, axenic cultures of Neocallimastix frontalis was performed using both fresh and air-dried perennial ryegrass leaf-blades of different cut-lengths. The methodology adopted removed the lag-phase and demonstrated the digestion of un-autoclaved leaf-blades. Fermentation of leaf-blades of 4.0 cm cut-length produced 18.4% more gas yet retained 11.2% more apparent DM relative to 0.5 cm cut-length leaf-blades. Drying did not affect fermentation by N. frontalis, although an interaction between drying and leaf-blade cut-length was noted. Removal of the lag phase and the use of un-autoclaved substrates are important when considering the biotechnological potential of anaerobic fungi. A hypothesis based upon sporulation at cut surfaces is proposed to describe the experimental results. MDPI 2020-11-11 /pmc/articles/PMC7696013/ /pubmed/33187375 http://dx.doi.org/10.3390/microorganisms8111774 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jimenez, Hugo R.
Edwards, Joan E.
Sanderson, Ruth
Kingston-Smith, Alison H.
McEwan, Neil R.
Theodorou, Michael K.
Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
title Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
title_full Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
title_fullStr Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
title_full_unstemmed Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
title_short Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
title_sort cut-lengths of perennial ryegrass leaf-blades influences in vitro fermentation by the anaerobic fungus neocallimastix frontalis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696013/
https://www.ncbi.nlm.nih.gov/pubmed/33187375
http://dx.doi.org/10.3390/microorganisms8111774
work_keys_str_mv AT jimenezhugor cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis
AT edwardsjoane cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis
AT sandersonruth cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis
AT kingstonsmithalisonh cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis
AT mcewanneilr cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis
AT theodoroumichaelk cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis