Cargando…
Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis
Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696013/ https://www.ncbi.nlm.nih.gov/pubmed/33187375 http://dx.doi.org/10.3390/microorganisms8111774 |
_version_ | 1783615311222669312 |
---|---|
author | Jimenez, Hugo R. Edwards, Joan E. Sanderson, Ruth Kingston-Smith, Alison H. McEwan, Neil R. Theodorou, Michael K. |
author_facet | Jimenez, Hugo R. Edwards, Joan E. Sanderson, Ruth Kingston-Smith, Alison H. McEwan, Neil R. Theodorou, Michael K. |
author_sort | Jimenez, Hugo R. |
collection | PubMed |
description | Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown to affect the ability of anaerobic fungi to break down plant biomass. However, methods to reduce the non-productive lag time in batch cultures and the effect of leaf-blade cut-length and condition on the fungal fermentation are not known. Therefore, experimentation using a novel gas production approach with pre-grown, axenic cultures of Neocallimastix frontalis was performed using both fresh and air-dried perennial ryegrass leaf-blades of different cut-lengths. The methodology adopted removed the lag-phase and demonstrated the digestion of un-autoclaved leaf-blades. Fermentation of leaf-blades of 4.0 cm cut-length produced 18.4% more gas yet retained 11.2% more apparent DM relative to 0.5 cm cut-length leaf-blades. Drying did not affect fermentation by N. frontalis, although an interaction between drying and leaf-blade cut-length was noted. Removal of the lag phase and the use of un-autoclaved substrates are important when considering the biotechnological potential of anaerobic fungi. A hypothesis based upon sporulation at cut surfaces is proposed to describe the experimental results. |
format | Online Article Text |
id | pubmed-7696013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76960132020-11-29 Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis Jimenez, Hugo R. Edwards, Joan E. Sanderson, Ruth Kingston-Smith, Alison H. McEwan, Neil R. Theodorou, Michael K. Microorganisms Article Anaerobic fungi in the gut of domesticated and wild mammalian herbivores play a key role in the host’s ability to utilize plant biomass. Due to their highly effective ability to enzymatically degrade lignocellulose, anaerobic fungi are biotechnologically interesting. Numerous factors have been shown to affect the ability of anaerobic fungi to break down plant biomass. However, methods to reduce the non-productive lag time in batch cultures and the effect of leaf-blade cut-length and condition on the fungal fermentation are not known. Therefore, experimentation using a novel gas production approach with pre-grown, axenic cultures of Neocallimastix frontalis was performed using both fresh and air-dried perennial ryegrass leaf-blades of different cut-lengths. The methodology adopted removed the lag-phase and demonstrated the digestion of un-autoclaved leaf-blades. Fermentation of leaf-blades of 4.0 cm cut-length produced 18.4% more gas yet retained 11.2% more apparent DM relative to 0.5 cm cut-length leaf-blades. Drying did not affect fermentation by N. frontalis, although an interaction between drying and leaf-blade cut-length was noted. Removal of the lag phase and the use of un-autoclaved substrates are important when considering the biotechnological potential of anaerobic fungi. A hypothesis based upon sporulation at cut surfaces is proposed to describe the experimental results. MDPI 2020-11-11 /pmc/articles/PMC7696013/ /pubmed/33187375 http://dx.doi.org/10.3390/microorganisms8111774 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jimenez, Hugo R. Edwards, Joan E. Sanderson, Ruth Kingston-Smith, Alison H. McEwan, Neil R. Theodorou, Michael K. Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis |
title | Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis |
title_full | Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis |
title_fullStr | Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis |
title_full_unstemmed | Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis |
title_short | Cut-Lengths of Perennial Ryegrass Leaf-Blades Influences In Vitro Fermentation by the Anaerobic Fungus Neocallimastix frontalis |
title_sort | cut-lengths of perennial ryegrass leaf-blades influences in vitro fermentation by the anaerobic fungus neocallimastix frontalis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696013/ https://www.ncbi.nlm.nih.gov/pubmed/33187375 http://dx.doi.org/10.3390/microorganisms8111774 |
work_keys_str_mv | AT jimenezhugor cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis AT edwardsjoane cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis AT sandersonruth cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis AT kingstonsmithalisonh cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis AT mcewanneilr cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis AT theodoroumichaelk cutlengthsofperennialryegrassleafbladesinfluencesinvitrofermentationbytheanaerobicfungusneocallimastixfrontalis |