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Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae

Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infection...

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Autores principales: Kallas, Pawel, Haugen, Håvard J, Gadegaard, Nikolaj, Stormonth-Darling, John, Hulander, Mats, Andersson, Martin, Valen, Håkon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696039/
https://www.ncbi.nlm.nih.gov/pubmed/33198386
http://dx.doi.org/10.3390/nano10112247
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author Kallas, Pawel
Haugen, Håvard J
Gadegaard, Nikolaj
Stormonth-Darling, John
Hulander, Mats
Andersson, Martin
Valen, Håkon
author_facet Kallas, Pawel
Haugen, Håvard J
Gadegaard, Nikolaj
Stormonth-Darling, John
Hulander, Mats
Andersson, Martin
Valen, Håkon
author_sort Kallas, Pawel
collection PubMed
description Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the E. coli wild type BW25113 and ΔfimA (with a knockout of major pilus protein FimA) and ΔfimH (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the E. coli wild type, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.
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spelling pubmed-76960392020-11-29 Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae Kallas, Pawel Haugen, Håvard J Gadegaard, Nikolaj Stormonth-Darling, John Hulander, Mats Andersson, Martin Valen, Håkon Nanomaterials (Basel) Article Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the E. coli wild type BW25113 and ΔfimA (with a knockout of major pilus protein FimA) and ΔfimH (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the E. coli wild type, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants. MDPI 2020-11-12 /pmc/articles/PMC7696039/ /pubmed/33198386 http://dx.doi.org/10.3390/nano10112247 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kallas, Pawel
Haugen, Håvard J
Gadegaard, Nikolaj
Stormonth-Darling, John
Hulander, Mats
Andersson, Martin
Valen, Håkon
Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
title Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
title_full Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
title_fullStr Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
title_full_unstemmed Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
title_short Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
title_sort adhesion of escherichia coli to nanostructured surfaces and the role of type 1 fimbriae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696039/
https://www.ncbi.nlm.nih.gov/pubmed/33198386
http://dx.doi.org/10.3390/nano10112247
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