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Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia

Neurological dementias such as Alzheimer’s disease and Lewy body dementia are thought to be caused in part by the formation and deposition of characteristic insoluble fibrils of polypeptides such as amyloid beta (Aβ), Tau, and/or α-synuclein (αSyn). In this context, it is critical to suppress and re...

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Autores principales: Ogawa, Kenjirou, Ishii, Ayumi, Shindo, Aimi, Hongo, Kunihiro, Mizobata, Tomohiro, Sogon, Tetsuya, Kawata, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696425/
https://www.ncbi.nlm.nih.gov/pubmed/33202830
http://dx.doi.org/10.3390/nu12113480
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author Ogawa, Kenjirou
Ishii, Ayumi
Shindo, Aimi
Hongo, Kunihiro
Mizobata, Tomohiro
Sogon, Tetsuya
Kawata, Yasushi
author_facet Ogawa, Kenjirou
Ishii, Ayumi
Shindo, Aimi
Hongo, Kunihiro
Mizobata, Tomohiro
Sogon, Tetsuya
Kawata, Yasushi
author_sort Ogawa, Kenjirou
collection PubMed
description Neurological dementias such as Alzheimer’s disease and Lewy body dementia are thought to be caused in part by the formation and deposition of characteristic insoluble fibrils of polypeptides such as amyloid beta (Aβ), Tau, and/or α-synuclein (αSyn). In this context, it is critical to suppress and remove such aggregates in order to prevent and/or delay the progression of dementia in these ailments. In this report, we investigated the effects of spearmint extract (SME) and rosmarinic acid (RA; the major component of SME) on the amyloid fibril formation reactions of αSyn, Aβ, and Tau proteins in vitro. SME or RA was added to soluble samples of each protein and the formation of fibrils was monitored by thioflavin T (ThioT) binding assays and transmission electron microscopy (TEM). We also evaluated whether preformed amyloid fibrils could be dissolved by the addition of RA. Our results reveal for the first time that SME and RA both suppress amyloid fibril formation, and that RA could disassemble preformed fibrils of αSyn, Aβ, and Tau into non-toxic species. Our results suggest that SME and RA may potentially suppress amyloid fibrils implicated in the progression of Alzheimer’s disease and Lewy body dementia in vivo, as well.
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spelling pubmed-76964252020-11-29 Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia Ogawa, Kenjirou Ishii, Ayumi Shindo, Aimi Hongo, Kunihiro Mizobata, Tomohiro Sogon, Tetsuya Kawata, Yasushi Nutrients Article Neurological dementias such as Alzheimer’s disease and Lewy body dementia are thought to be caused in part by the formation and deposition of characteristic insoluble fibrils of polypeptides such as amyloid beta (Aβ), Tau, and/or α-synuclein (αSyn). In this context, it is critical to suppress and remove such aggregates in order to prevent and/or delay the progression of dementia in these ailments. In this report, we investigated the effects of spearmint extract (SME) and rosmarinic acid (RA; the major component of SME) on the amyloid fibril formation reactions of αSyn, Aβ, and Tau proteins in vitro. SME or RA was added to soluble samples of each protein and the formation of fibrils was monitored by thioflavin T (ThioT) binding assays and transmission electron microscopy (TEM). We also evaluated whether preformed amyloid fibrils could be dissolved by the addition of RA. Our results reveal for the first time that SME and RA both suppress amyloid fibril formation, and that RA could disassemble preformed fibrils of αSyn, Aβ, and Tau into non-toxic species. Our results suggest that SME and RA may potentially suppress amyloid fibrils implicated in the progression of Alzheimer’s disease and Lewy body dementia in vivo, as well. MDPI 2020-11-13 /pmc/articles/PMC7696425/ /pubmed/33202830 http://dx.doi.org/10.3390/nu12113480 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ogawa, Kenjirou
Ishii, Ayumi
Shindo, Aimi
Hongo, Kunihiro
Mizobata, Tomohiro
Sogon, Tetsuya
Kawata, Yasushi
Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
title Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
title_full Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
title_fullStr Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
title_full_unstemmed Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
title_short Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
title_sort spearmint extract containing rosmarinic acid suppresses amyloid fibril formation of proteins associated with dementia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696425/
https://www.ncbi.nlm.nih.gov/pubmed/33202830
http://dx.doi.org/10.3390/nu12113480
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