Cargando…
Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes
Willaertia magna C2c Maky is a free-living amoeba that has demonstrated its ability to inhibit the intracellular multiplication of some Legionella pneumophila strains, which are pathogenic bacteria inhabiting the aquatic environment. The Amoeba, an industry involved in the treatment of microbiologic...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696429/ https://www.ncbi.nlm.nih.gov/pubmed/33207645 http://dx.doi.org/10.3390/microorganisms8111791 |
_version_ | 1783615402031448064 |
---|---|
author | Hasni, Issam Armstrong, Nicholas Decloquement, Philippe Azza, Said Fontanini, Anthony Abbe, Olivier Cherif Louazani, Amina Demanèche, Sandrine Chabrière, Eric Colson, Philippe La Scola, Bernard |
author_facet | Hasni, Issam Armstrong, Nicholas Decloquement, Philippe Azza, Said Fontanini, Anthony Abbe, Olivier Cherif Louazani, Amina Demanèche, Sandrine Chabrière, Eric Colson, Philippe La Scola, Bernard |
author_sort | Hasni, Issam |
collection | PubMed |
description | Willaertia magna C2c Maky is a free-living amoeba that has demonstrated its ability to inhibit the intracellular multiplication of some Legionella pneumophila strains, which are pathogenic bacteria inhabiting the aquatic environment. The Amoeba, an industry involved in the treatment of microbiological risk in the water and plant protection sectors, has developed a natural biocide based on the property of W. magna to manage the proliferation of the pathogen in cooling towers. In axenic liquid medium, amoebas are usually cultivated in adhesion on culture flask. However, we implemented a liquid culture in suspension using bioreactors in order to produce large quantities of W. magna. In order to investigate the culture condition effects on W. magna, we conducted a study based on microscopic, proteomics and lipidomics analyzes. According to the culture condition, amoeba exhibited two different phenotypes. The differential proteomics study showed that amoebas seemed to promote the lipid metabolism pathway in suspension culture, whereas we observed an upregulation of the carbohydrate pathway in adherent culture. Furthermore, we observed an over-regulation of proteins related to the cytoskeleton for W. magna cells grown in adhesion. Regarding the lipid analysis, suspension and adhesion cell growth showed comparable lipid class compositions. However, the differential lipid analysis revealed differences that confirmed cell phenotype differences observed by microscopy and predicted by proteomics. Overall, this study provides us with a better insight into the biology and molecular processes of W. magna in different culture lifestyles. |
format | Online Article Text |
id | pubmed-7696429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76964292020-11-29 Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes Hasni, Issam Armstrong, Nicholas Decloquement, Philippe Azza, Said Fontanini, Anthony Abbe, Olivier Cherif Louazani, Amina Demanèche, Sandrine Chabrière, Eric Colson, Philippe La Scola, Bernard Microorganisms Article Willaertia magna C2c Maky is a free-living amoeba that has demonstrated its ability to inhibit the intracellular multiplication of some Legionella pneumophila strains, which are pathogenic bacteria inhabiting the aquatic environment. The Amoeba, an industry involved in the treatment of microbiological risk in the water and plant protection sectors, has developed a natural biocide based on the property of W. magna to manage the proliferation of the pathogen in cooling towers. In axenic liquid medium, amoebas are usually cultivated in adhesion on culture flask. However, we implemented a liquid culture in suspension using bioreactors in order to produce large quantities of W. magna. In order to investigate the culture condition effects on W. magna, we conducted a study based on microscopic, proteomics and lipidomics analyzes. According to the culture condition, amoeba exhibited two different phenotypes. The differential proteomics study showed that amoebas seemed to promote the lipid metabolism pathway in suspension culture, whereas we observed an upregulation of the carbohydrate pathway in adherent culture. Furthermore, we observed an over-regulation of proteins related to the cytoskeleton for W. magna cells grown in adhesion. Regarding the lipid analysis, suspension and adhesion cell growth showed comparable lipid class compositions. However, the differential lipid analysis revealed differences that confirmed cell phenotype differences observed by microscopy and predicted by proteomics. Overall, this study provides us with a better insight into the biology and molecular processes of W. magna in different culture lifestyles. MDPI 2020-11-16 /pmc/articles/PMC7696429/ /pubmed/33207645 http://dx.doi.org/10.3390/microorganisms8111791 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hasni, Issam Armstrong, Nicholas Decloquement, Philippe Azza, Said Fontanini, Anthony Abbe, Olivier Cherif Louazani, Amina Demanèche, Sandrine Chabrière, Eric Colson, Philippe La Scola, Bernard Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes |
title | Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes |
title_full | Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes |
title_fullStr | Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes |
title_full_unstemmed | Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes |
title_short | Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes |
title_sort | proteomics and lipidomics investigations to decipher the behavior of willaertia magna c2c maky according to different culture modes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696429/ https://www.ncbi.nlm.nih.gov/pubmed/33207645 http://dx.doi.org/10.3390/microorganisms8111791 |
work_keys_str_mv | AT hasniissam proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT armstrongnicholas proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT decloquementphilippe proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT azzasaid proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT fontaninianthony proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT abbeolivier proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT cheriflouazaniamina proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT demanechesandrine proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT chabriereeric proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT colsonphilippe proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes AT lascolabernard proteomicsandlipidomicsinvestigationstodecipherthebehaviorofwillaertiamagnac2cmakyaccordingtodifferentculturemodes |