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Enzymatic Responses to Low-Intensity Radiation of Tritium

The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as...

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Autores principales: Rozhko, Tatiana V., Nemtseva, Elena V., Gardt, Maria V., Raikov, Alexander V., Lisitsa, Albert E., Badun, Gennadii A., Kudryasheva, Nadezhda S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696592/
https://www.ncbi.nlm.nih.gov/pubmed/33187108
http://dx.doi.org/10.3390/ijms21228464
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author Rozhko, Tatiana V.
Nemtseva, Elena V.
Gardt, Maria V.
Raikov, Alexander V.
Lisitsa, Albert E.
Badun, Gennadii A.
Kudryasheva, Nadezhda S.
author_facet Rozhko, Tatiana V.
Nemtseva, Elena V.
Gardt, Maria V.
Raikov, Alexander V.
Lisitsa, Albert E.
Badun, Gennadii A.
Kudryasheva, Nadezhda S.
author_sort Rozhko, Tatiana V.
collection PubMed
description The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon.
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spelling pubmed-76965922020-11-29 Enzymatic Responses to Low-Intensity Radiation of Tritium Rozhko, Tatiana V. Nemtseva, Elena V. Gardt, Maria V. Raikov, Alexander V. Lisitsa, Albert E. Badun, Gennadii A. Kudryasheva, Nadezhda S. Int J Mol Sci Article The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon. MDPI 2020-11-11 /pmc/articles/PMC7696592/ /pubmed/33187108 http://dx.doi.org/10.3390/ijms21228464 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rozhko, Tatiana V.
Nemtseva, Elena V.
Gardt, Maria V.
Raikov, Alexander V.
Lisitsa, Albert E.
Badun, Gennadii A.
Kudryasheva, Nadezhda S.
Enzymatic Responses to Low-Intensity Radiation of Tritium
title Enzymatic Responses to Low-Intensity Radiation of Tritium
title_full Enzymatic Responses to Low-Intensity Radiation of Tritium
title_fullStr Enzymatic Responses to Low-Intensity Radiation of Tritium
title_full_unstemmed Enzymatic Responses to Low-Intensity Radiation of Tritium
title_short Enzymatic Responses to Low-Intensity Radiation of Tritium
title_sort enzymatic responses to low-intensity radiation of tritium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696592/
https://www.ncbi.nlm.nih.gov/pubmed/33187108
http://dx.doi.org/10.3390/ijms21228464
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