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Enzymatic Responses to Low-Intensity Radiation of Tritium
The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696592/ https://www.ncbi.nlm.nih.gov/pubmed/33187108 http://dx.doi.org/10.3390/ijms21228464 |
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author | Rozhko, Tatiana V. Nemtseva, Elena V. Gardt, Maria V. Raikov, Alexander V. Lisitsa, Albert E. Badun, Gennadii A. Kudryasheva, Nadezhda S. |
author_facet | Rozhko, Tatiana V. Nemtseva, Elena V. Gardt, Maria V. Raikov, Alexander V. Lisitsa, Albert E. Badun, Gennadii A. Kudryasheva, Nadezhda S. |
author_sort | Rozhko, Tatiana V. |
collection | PubMed |
description | The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon. |
format | Online Article Text |
id | pubmed-7696592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76965922020-11-29 Enzymatic Responses to Low-Intensity Radiation of Tritium Rozhko, Tatiana V. Nemtseva, Elena V. Gardt, Maria V. Raikov, Alexander V. Lisitsa, Albert E. Badun, Gennadii A. Kudryasheva, Nadezhda S. Int J Mol Sci Article The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon. MDPI 2020-11-11 /pmc/articles/PMC7696592/ /pubmed/33187108 http://dx.doi.org/10.3390/ijms21228464 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rozhko, Tatiana V. Nemtseva, Elena V. Gardt, Maria V. Raikov, Alexander V. Lisitsa, Albert E. Badun, Gennadii A. Kudryasheva, Nadezhda S. Enzymatic Responses to Low-Intensity Radiation of Tritium |
title | Enzymatic Responses to Low-Intensity Radiation of Tritium |
title_full | Enzymatic Responses to Low-Intensity Radiation of Tritium |
title_fullStr | Enzymatic Responses to Low-Intensity Radiation of Tritium |
title_full_unstemmed | Enzymatic Responses to Low-Intensity Radiation of Tritium |
title_short | Enzymatic Responses to Low-Intensity Radiation of Tritium |
title_sort | enzymatic responses to low-intensity radiation of tritium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696592/ https://www.ncbi.nlm.nih.gov/pubmed/33187108 http://dx.doi.org/10.3390/ijms21228464 |
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