Effect of Heat Stress on Dairy Cow Performance and on Expression of Protein Metabolism Genes in Mammary Cells

SIMPLE SUMMARY: Environmental temperatures are increasing, and consequent global warming also has negative effects on dairy cattle farms, which may result in reduced production and poorer milk quality. The protein content of casein, in particular, is important in influencing the coagulation properties of milk and, therefore, the production and quality of cheese. The aim of this study was to assess the effect of heat stress on animal performance and on the expression of selected genes involved in milk protein metabolism. Eight dairy cows were kept under thermoneutral conditions for 8 days. The same animals were then maintained under mild heat stress conditions for an additional 8 days. The results of this study revealed that mild heat stress reduced the feed intake and performance of dairy cows in terms of milk and protein yield, but not the expression of the target genes involved in milk protein metabolism, such as those coding for caseins. ABSTRACT: The aim of this study was to assess the effect of heat stress on dairy cow performance and on the expression of selected genes involved in milk protein metabolism. Eight Italian Holstein Friesian cows were kept under thermoneutral conditions (temperature–humidity index (THI) < 72, CON) for 8 days and under mild heat stress conditions (72 < THI < 78, HS) for an additional 8 days. The rectal temperature, feed intake, and milk yield were recorded during the last 3 days of the CON and HS periods. During the same time period, milk samples were collected to assess the composition and expression of selected genes involved in milk protein metabolism. Gene expression analyses were performed on somatic cells from milk, which are representative of mammary tissue. In terms of dairy cow performance, HS resulted in lower milk and protein yields and feed intake but higher rectal temperature than for CON (p < 0.05). Under HS, there were greater abundances of HSPA1A (p < 0.05) and BCL2 (p < 0.05), compared to CON, but similar levels of CSN2 (p > 0.05), CSN3 (p > 0.05), HSPA8 (p > 0.05), and STAT5B (p > 0.05) mRNA. Mild heat stress reduced the performance of dairy cows without affecting the expression of genes coding for caseins.