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Advances in Optical Detection of Human-Associated Pathogenic Bacteria
Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696695/ https://www.ncbi.nlm.nih.gov/pubmed/33187331 http://dx.doi.org/10.3390/molecules25225256 |
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author | Locke, Andrea Fitzgerald, Sean Mahadevan-Jansen, Anita |
author_facet | Locke, Andrea Fitzgerald, Sean Mahadevan-Jansen, Anita |
author_sort | Locke, Andrea |
collection | PubMed |
description | Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a culture-free environment. Current diagnostic techniques lack speed and effectiveness in detecting bacteria that are culture-negative, as well as options for in vivo detection. The optical detection of bacteria offers the potential to overcome these obstacles by providing various platforms that can detect bacteria rapidly, with minimum sample preparation, and, in some cases, culture-free directly from patient fluids or even in vivo. These modalities include infrared, Raman, and fluorescence spectroscopy, along with optical coherence tomography, interference, polarization, and laser speckle. However, these techniques are not without their own set of limitations. This review summarizes the strengths and weaknesses of utilizing each of these optical tools for rapid bacteria detection and identification. |
format | Online Article Text |
id | pubmed-7696695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76966952020-11-29 Advances in Optical Detection of Human-Associated Pathogenic Bacteria Locke, Andrea Fitzgerald, Sean Mahadevan-Jansen, Anita Molecules Review Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a culture-free environment. Current diagnostic techniques lack speed and effectiveness in detecting bacteria that are culture-negative, as well as options for in vivo detection. The optical detection of bacteria offers the potential to overcome these obstacles by providing various platforms that can detect bacteria rapidly, with minimum sample preparation, and, in some cases, culture-free directly from patient fluids or even in vivo. These modalities include infrared, Raman, and fluorescence spectroscopy, along with optical coherence tomography, interference, polarization, and laser speckle. However, these techniques are not without their own set of limitations. This review summarizes the strengths and weaknesses of utilizing each of these optical tools for rapid bacteria detection and identification. MDPI 2020-11-11 /pmc/articles/PMC7696695/ /pubmed/33187331 http://dx.doi.org/10.3390/molecules25225256 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Locke, Andrea Fitzgerald, Sean Mahadevan-Jansen, Anita Advances in Optical Detection of Human-Associated Pathogenic Bacteria |
title | Advances in Optical Detection of Human-Associated Pathogenic Bacteria |
title_full | Advances in Optical Detection of Human-Associated Pathogenic Bacteria |
title_fullStr | Advances in Optical Detection of Human-Associated Pathogenic Bacteria |
title_full_unstemmed | Advances in Optical Detection of Human-Associated Pathogenic Bacteria |
title_short | Advances in Optical Detection of Human-Associated Pathogenic Bacteria |
title_sort | advances in optical detection of human-associated pathogenic bacteria |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696695/ https://www.ncbi.nlm.nih.gov/pubmed/33187331 http://dx.doi.org/10.3390/molecules25225256 |
work_keys_str_mv | AT lockeandrea advancesinopticaldetectionofhumanassociatedpathogenicbacteria AT fitzgeraldsean advancesinopticaldetectionofhumanassociatedpathogenicbacteria AT mahadevanjansenanita advancesinopticaldetectionofhumanassociatedpathogenicbacteria |