Cargando…

Advances in Optical Detection of Human-Associated Pathogenic Bacteria

Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a...

Descripción completa

Detalles Bibliográficos
Autores principales: Locke, Andrea, Fitzgerald, Sean, Mahadevan-Jansen, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696695/
https://www.ncbi.nlm.nih.gov/pubmed/33187331
http://dx.doi.org/10.3390/molecules25225256
_version_ 1783615462576226304
author Locke, Andrea
Fitzgerald, Sean
Mahadevan-Jansen, Anita
author_facet Locke, Andrea
Fitzgerald, Sean
Mahadevan-Jansen, Anita
author_sort Locke, Andrea
collection PubMed
description Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a culture-free environment. Current diagnostic techniques lack speed and effectiveness in detecting bacteria that are culture-negative, as well as options for in vivo detection. The optical detection of bacteria offers the potential to overcome these obstacles by providing various platforms that can detect bacteria rapidly, with minimum sample preparation, and, in some cases, culture-free directly from patient fluids or even in vivo. These modalities include infrared, Raman, and fluorescence spectroscopy, along with optical coherence tomography, interference, polarization, and laser speckle. However, these techniques are not without their own set of limitations. This review summarizes the strengths and weaknesses of utilizing each of these optical tools for rapid bacteria detection and identification.
format Online
Article
Text
id pubmed-7696695
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76966952020-11-29 Advances in Optical Detection of Human-Associated Pathogenic Bacteria Locke, Andrea Fitzgerald, Sean Mahadevan-Jansen, Anita Molecules Review Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a culture-free environment. Current diagnostic techniques lack speed and effectiveness in detecting bacteria that are culture-negative, as well as options for in vivo detection. The optical detection of bacteria offers the potential to overcome these obstacles by providing various platforms that can detect bacteria rapidly, with minimum sample preparation, and, in some cases, culture-free directly from patient fluids or even in vivo. These modalities include infrared, Raman, and fluorescence spectroscopy, along with optical coherence tomography, interference, polarization, and laser speckle. However, these techniques are not without their own set of limitations. This review summarizes the strengths and weaknesses of utilizing each of these optical tools for rapid bacteria detection and identification. MDPI 2020-11-11 /pmc/articles/PMC7696695/ /pubmed/33187331 http://dx.doi.org/10.3390/molecules25225256 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Locke, Andrea
Fitzgerald, Sean
Mahadevan-Jansen, Anita
Advances in Optical Detection of Human-Associated Pathogenic Bacteria
title Advances in Optical Detection of Human-Associated Pathogenic Bacteria
title_full Advances in Optical Detection of Human-Associated Pathogenic Bacteria
title_fullStr Advances in Optical Detection of Human-Associated Pathogenic Bacteria
title_full_unstemmed Advances in Optical Detection of Human-Associated Pathogenic Bacteria
title_short Advances in Optical Detection of Human-Associated Pathogenic Bacteria
title_sort advances in optical detection of human-associated pathogenic bacteria
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696695/
https://www.ncbi.nlm.nih.gov/pubmed/33187331
http://dx.doi.org/10.3390/molecules25225256
work_keys_str_mv AT lockeandrea advancesinopticaldetectionofhumanassociatedpathogenicbacteria
AT fitzgeraldsean advancesinopticaldetectionofhumanassociatedpathogenicbacteria
AT mahadevanjansenanita advancesinopticaldetectionofhumanassociatedpathogenicbacteria