Novel Quantitative Analysis Using Optical Imaging (VELscope) and Spectroscopy (Raman) Techniques for Oral Cancer Detection

SIMPLE SUMMARY: Our study aims to develop a novel quantitative analysis method that can increase the oral cancer detection rate for screening oral cancer. We used two different optical techniques, a light-based detection technique (VELScope) and a vibrational spectroscopic technique (Raman spectroscopy). First, we analyzed and evaluated the performance of these two techniques individually using PCA–LDA, and PCA–QDA classifiers. The PCA–LDA of Raman spectroscopy had 82.9% accuracy, 80% sensitivity, and 85.7% specificity, while the region of interests on the autofluorescence images were differentiated with 90% accuracy, 100% sensitivity, and 80% specificity. Afterward, we combined both techniques and evaluated their performance. The combination of two optical techniques can differentiate the cancer and normal groups with 97.14% accuracy, 100% sensitivity, and 94.3% specificity. The main advantage of our study is that we can confirm our results by using two different techniques that are completely independent of each other. That is the reason that the combination of two techniques can increase the sensitivity and specificity. ABSTRACT: In this study, we developed a novel quantitative analysis method to enhance the detection capability for oral cancer screening. We combined two different optical techniques, a light-based detection technique (visually enhanced lesion scope) and a vibrational spectroscopic technique (Raman spectroscopy). Materials and methods: Thirty-five oral cancer patients who went through surgery were enrolled. Thirty-five cancer lesions and thirty-five control samples with normal oral mucosa (adjacent to the cancer lesion) were analyzed. Thirty-five autofluorescence images and 70 Raman spectra were taken from 35 cancer and 35 control group cryopreserved samples. The normalized intensity and heterogeneity of the 70 regions of interest (ROIs) were calculated along with 70 averaged Raman spectra. Linear discriminant analysis (LDA) and quadratic discriminant analysis (QDA) were used with principal component analysis (PCA) to differentiate the cancer and control groups (normal). The classifications rates were validated using two different validation methods, leave-one-out cross-validation (LOOCV) and k-fold cross-validation. Results: The cryopreserved normal and tumor tissues were differentiated using the PCA–LDA and PCA–QDA models. The PCA–LDA of Raman spectroscopy (RS) had 82.9% accuracy, 80% sensitivity, and 85.7% specificity, while ROIs on the autofluorescence images were differentiated with 90% accuracy, 100% sensitivity, and 80% specificity. The combination of two optical techniques differentiated cancer and normal group with 97.14% accuracy, 100% sensitivity, and 94.3% specificity. Conclusion: In this study, we combined the data of two different optical techniques. Furthermore, PCA–LDA and PCA–QDA quantitative analysis models were used to differentiate tumor and normal groups, creating a complementary pathway for efficient tumor diagnosis. The error rates of RS and VELcope analysis were 17.10% and 10%, respectively, which was reduced to 3% when the two optical techniques were combined.