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Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation

The Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) is characterized by the infiltration of lymphocytes and a unique tumor microenvironment. Exosomes from cancer cells are essential for intercellular communication. The aims of this study were to investigate the secretion of EBVaGC exo...

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Autores principales: Hinata, Munetoshi, Kunita, Akiko, Abe, Hiroyuki, Morishita, Yasuyuki, Sakuma, Kei, Yamashita, Hiroharu, Seto, Yasuyuki, Ushiku, Tetsuo, Fukayama, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697542/
https://www.ncbi.nlm.nih.gov/pubmed/33198173
http://dx.doi.org/10.3390/microorganisms8111776
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author Hinata, Munetoshi
Kunita, Akiko
Abe, Hiroyuki
Morishita, Yasuyuki
Sakuma, Kei
Yamashita, Hiroharu
Seto, Yasuyuki
Ushiku, Tetsuo
Fukayama, Masashi
author_facet Hinata, Munetoshi
Kunita, Akiko
Abe, Hiroyuki
Morishita, Yasuyuki
Sakuma, Kei
Yamashita, Hiroharu
Seto, Yasuyuki
Ushiku, Tetsuo
Fukayama, Masashi
author_sort Hinata, Munetoshi
collection PubMed
description The Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) is characterized by the infiltration of lymphocytes and a unique tumor microenvironment. Exosomes from cancer cells are essential for intercellular communication. The aims of this study were to investigate the secretion of EBVaGC exosomes and their physiological effect on dendritic cell maturation in vitro and to characterize dendritic cells (DCs) in EBVaGC in vivo. Western blotting analysis of CD63 and CD81 of exosomes from EBV-infected gastric cancer cell lines indicated an increase in exosome secretion. The fraction of monocyte-derived DCs positive for the maturation marker CD86 was significantly suppressed when incubated with exosomes from EBV-infected gastric cancer cell lines. Immunohistochemical analysis of GC tissues expressing DC markers (S100, Langerin, CD1a, CD83, CD86, and BDCA-2) indicated that the density of DCs was generally higher in EBVaGC than in EBV-negative GC, although the numbers of CD83- and CD86-positive DCs were decreased in the group with high numbers of CD1a-positive DCs. A low number of CD83-positive DCs was marginally correlated with worse prognosis of EBVaGC in patients. EBVaGC is a tumor with abundant DCs, including immature and mature DCs. Moreover, the maturation of DCs is suppressed by exosomes from EBV-infected epithelial cells.
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spelling pubmed-76975422020-11-29 Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation Hinata, Munetoshi Kunita, Akiko Abe, Hiroyuki Morishita, Yasuyuki Sakuma, Kei Yamashita, Hiroharu Seto, Yasuyuki Ushiku, Tetsuo Fukayama, Masashi Microorganisms Article The Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) is characterized by the infiltration of lymphocytes and a unique tumor microenvironment. Exosomes from cancer cells are essential for intercellular communication. The aims of this study were to investigate the secretion of EBVaGC exosomes and their physiological effect on dendritic cell maturation in vitro and to characterize dendritic cells (DCs) in EBVaGC in vivo. Western blotting analysis of CD63 and CD81 of exosomes from EBV-infected gastric cancer cell lines indicated an increase in exosome secretion. The fraction of monocyte-derived DCs positive for the maturation marker CD86 was significantly suppressed when incubated with exosomes from EBV-infected gastric cancer cell lines. Immunohistochemical analysis of GC tissues expressing DC markers (S100, Langerin, CD1a, CD83, CD86, and BDCA-2) indicated that the density of DCs was generally higher in EBVaGC than in EBV-negative GC, although the numbers of CD83- and CD86-positive DCs were decreased in the group with high numbers of CD1a-positive DCs. A low number of CD83-positive DCs was marginally correlated with worse prognosis of EBVaGC in patients. EBVaGC is a tumor with abundant DCs, including immature and mature DCs. Moreover, the maturation of DCs is suppressed by exosomes from EBV-infected epithelial cells. MDPI 2020-11-12 /pmc/articles/PMC7697542/ /pubmed/33198173 http://dx.doi.org/10.3390/microorganisms8111776 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hinata, Munetoshi
Kunita, Akiko
Abe, Hiroyuki
Morishita, Yasuyuki
Sakuma, Kei
Yamashita, Hiroharu
Seto, Yasuyuki
Ushiku, Tetsuo
Fukayama, Masashi
Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation
title Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation
title_full Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation
title_fullStr Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation
title_full_unstemmed Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation
title_short Exosomes of Epstein-Barr Virus-Associated Gastric Carcinoma Suppress Dendritic Cell Maturation
title_sort exosomes of epstein-barr virus-associated gastric carcinoma suppress dendritic cell maturation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697542/
https://www.ncbi.nlm.nih.gov/pubmed/33198173
http://dx.doi.org/10.3390/microorganisms8111776
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