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Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles

Shallot landraces and varieties are considered an important genetic resource for Allium breeding due to their high contents of several functional metabolites. Aiming to provide new genetic materials for the development of a novel bulb onion cultivar derived from intraspecific hybrids with useful agr...

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Autores principales: Abdelrahman, Mostafa, Ariyanti, Nur Aeni, Sawada, Yuji, Tsuji, Fumitada, Hirata, Sho, Hang, Tran Thi Minh, Okamoto, Mami, Yamada, Yutaka, Tsugawa, Hiroshi, Hirai, Masami Yokota, Shigyo, Masayoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697566/
https://www.ncbi.nlm.nih.gov/pubmed/33202886
http://dx.doi.org/10.3390/molecules25225300
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author Abdelrahman, Mostafa
Ariyanti, Nur Aeni
Sawada, Yuji
Tsuji, Fumitada
Hirata, Sho
Hang, Tran Thi Minh
Okamoto, Mami
Yamada, Yutaka
Tsugawa, Hiroshi
Hirai, Masami Yokota
Shigyo, Masayoshi
author_facet Abdelrahman, Mostafa
Ariyanti, Nur Aeni
Sawada, Yuji
Tsuji, Fumitada
Hirata, Sho
Hang, Tran Thi Minh
Okamoto, Mami
Yamada, Yutaka
Tsugawa, Hiroshi
Hirai, Masami Yokota
Shigyo, Masayoshi
author_sort Abdelrahman, Mostafa
collection PubMed
description Shallot landraces and varieties are considered an important genetic resource for Allium breeding due to their high contents of several functional metabolites. Aiming to provide new genetic materials for the development of a novel bulb onion cultivar derived from intraspecific hybrids with useful agronomic traits from shallots, the metabolic profiles in the bulbs of 8 Indonesian shallot landraces and 7 short-day and 3 long-day bulb onion cultivars were established using LC–Q-TOF-MS/MS. Principal component analysis, partial least squares discriminant analysis, and dendrogram clustering analysis showed two major groups; group I contained all shallot landraces and group II contained all bulb onion cultivars, indicating that shallots exhibited a distinct metabolic profile in comparison with bulb onions. Variable importance in the projection and Spearman’s rank correlation indicated that free and conjugated amino acids, flavonoids (especially metabolites having flavonol aglycone), and anthocyanins, as well as organic acids, were among the top metabolite variables that were highly associated with shallot landraces. The absolute quantification of 21 amino acids using conventional HPLC analysis showed high contents in shallots rather than in bulb onions. The present study indicated that shallots reprogrammed their metabolism toward a high accumulation of amino acids and flavonoids as an adaptive mechanism in extremely hot tropical environments.
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spelling pubmed-76975662020-11-29 Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles Abdelrahman, Mostafa Ariyanti, Nur Aeni Sawada, Yuji Tsuji, Fumitada Hirata, Sho Hang, Tran Thi Minh Okamoto, Mami Yamada, Yutaka Tsugawa, Hiroshi Hirai, Masami Yokota Shigyo, Masayoshi Molecules Article Shallot landraces and varieties are considered an important genetic resource for Allium breeding due to their high contents of several functional metabolites. Aiming to provide new genetic materials for the development of a novel bulb onion cultivar derived from intraspecific hybrids with useful agronomic traits from shallots, the metabolic profiles in the bulbs of 8 Indonesian shallot landraces and 7 short-day and 3 long-day bulb onion cultivars were established using LC–Q-TOF-MS/MS. Principal component analysis, partial least squares discriminant analysis, and dendrogram clustering analysis showed two major groups; group I contained all shallot landraces and group II contained all bulb onion cultivars, indicating that shallots exhibited a distinct metabolic profile in comparison with bulb onions. Variable importance in the projection and Spearman’s rank correlation indicated that free and conjugated amino acids, flavonoids (especially metabolites having flavonol aglycone), and anthocyanins, as well as organic acids, were among the top metabolite variables that were highly associated with shallot landraces. The absolute quantification of 21 amino acids using conventional HPLC analysis showed high contents in shallots rather than in bulb onions. The present study indicated that shallots reprogrammed their metabolism toward a high accumulation of amino acids and flavonoids as an adaptive mechanism in extremely hot tropical environments. MDPI 2020-11-13 /pmc/articles/PMC7697566/ /pubmed/33202886 http://dx.doi.org/10.3390/molecules25225300 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdelrahman, Mostafa
Ariyanti, Nur Aeni
Sawada, Yuji
Tsuji, Fumitada
Hirata, Sho
Hang, Tran Thi Minh
Okamoto, Mami
Yamada, Yutaka
Tsugawa, Hiroshi
Hirai, Masami Yokota
Shigyo, Masayoshi
Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles
title Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles
title_full Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles
title_fullStr Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles
title_full_unstemmed Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles
title_short Metabolome-Based Discrimination Analysis of Shallot Landraces and Bulb Onion Cultivars Associated with Differences in the Amino Acid and Flavonoid Profiles
title_sort metabolome-based discrimination analysis of shallot landraces and bulb onion cultivars associated with differences in the amino acid and flavonoid profiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697566/
https://www.ncbi.nlm.nih.gov/pubmed/33202886
http://dx.doi.org/10.3390/molecules25225300
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