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Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens

Oral lichen planus (OLP) is a common chronic inflammatory disease affecting the oral mucosa. The pathogenesis of OLP is incompletely understood but is thought to be related to the immune system. As the oral cavity is a major reservoir and transmission gateway for bacteria, viruses, and fungi, the mi...

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Autores principales: Zhong, Evelyn F., Chang, Andrea, Stucky, Andres, Chen, Xuelian, Mundluru, Tarun, Khalifeh, Mohammad, Sedghizadeh, Parish P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697643/
https://www.ncbi.nlm.nih.gov/pubmed/33207582
http://dx.doi.org/10.3390/pathogens9110952
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author Zhong, Evelyn F.
Chang, Andrea
Stucky, Andres
Chen, Xuelian
Mundluru, Tarun
Khalifeh, Mohammad
Sedghizadeh, Parish P.
author_facet Zhong, Evelyn F.
Chang, Andrea
Stucky, Andres
Chen, Xuelian
Mundluru, Tarun
Khalifeh, Mohammad
Sedghizadeh, Parish P.
author_sort Zhong, Evelyn F.
collection PubMed
description Oral lichen planus (OLP) is a common chronic inflammatory disease affecting the oral mucosa. The pathogenesis of OLP is incompletely understood but is thought to be related to the immune system. As the oral cavity is a major reservoir and transmission gateway for bacteria, viruses, and fungi, the microbial composition of the oral cavity could play a role in the pathogenesis of OLP. However, limited by analytic technology and knowledge of the microbial community in the oral cavity, it is not yet clear which pathogens are associated with OLP. Next generation sequencing (NGS) is a powerful tool to identify pathogens for many infectious diseases. In this study, we compared the host cell gene expression profiles and the microbial profiles between OLP patients and matched healthy individuals. We identified the activation of the hepatocyte nuclear factor alpha (HNF4A) network in OLP patients and potential pathogens, including Corynebacterium matruchotii, Fusobacterium periodonticum, Streptococcus intermedius, Streptococcus oralis, and Prevotella denticola. Prevotella denticola is capable of activating the HNF4A gene network. Our findings shed light on the previously elusive association of OLP with various diseases like hepatitis, and indicate that OLP is a T-helper type 17 (Th17) mediated mucosal inflammatory process. The identified molecular pathways and microbes could be used to inform future investigations into OLP pathogenesis and to develop novel therapeutics for OLP treatment.
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spelling pubmed-76976432020-11-29 Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens Zhong, Evelyn F. Chang, Andrea Stucky, Andres Chen, Xuelian Mundluru, Tarun Khalifeh, Mohammad Sedghizadeh, Parish P. Pathogens Article Oral lichen planus (OLP) is a common chronic inflammatory disease affecting the oral mucosa. The pathogenesis of OLP is incompletely understood but is thought to be related to the immune system. As the oral cavity is a major reservoir and transmission gateway for bacteria, viruses, and fungi, the microbial composition of the oral cavity could play a role in the pathogenesis of OLP. However, limited by analytic technology and knowledge of the microbial community in the oral cavity, it is not yet clear which pathogens are associated with OLP. Next generation sequencing (NGS) is a powerful tool to identify pathogens for many infectious diseases. In this study, we compared the host cell gene expression profiles and the microbial profiles between OLP patients and matched healthy individuals. We identified the activation of the hepatocyte nuclear factor alpha (HNF4A) network in OLP patients and potential pathogens, including Corynebacterium matruchotii, Fusobacterium periodonticum, Streptococcus intermedius, Streptococcus oralis, and Prevotella denticola. Prevotella denticola is capable of activating the HNF4A gene network. Our findings shed light on the previously elusive association of OLP with various diseases like hepatitis, and indicate that OLP is a T-helper type 17 (Th17) mediated mucosal inflammatory process. The identified molecular pathways and microbes could be used to inform future investigations into OLP pathogenesis and to develop novel therapeutics for OLP treatment. MDPI 2020-11-16 /pmc/articles/PMC7697643/ /pubmed/33207582 http://dx.doi.org/10.3390/pathogens9110952 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhong, Evelyn F.
Chang, Andrea
Stucky, Andres
Chen, Xuelian
Mundluru, Tarun
Khalifeh, Mohammad
Sedghizadeh, Parish P.
Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens
title Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens
title_full Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens
title_fullStr Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens
title_full_unstemmed Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens
title_short Genomic Analysis of Oral Lichen Planus and Related Oral Microbiome Pathogens
title_sort genomic analysis of oral lichen planus and related oral microbiome pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697643/
https://www.ncbi.nlm.nih.gov/pubmed/33207582
http://dx.doi.org/10.3390/pathogens9110952
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