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Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors
Small-cell lung cancer (SCLC) is an aggressive malignant cancer that is classified into four subtypes based on the expression of the following key transcription and co-transcription factors: ASCL1, NEUROD1, YAP1, and POU2F3. The protein expression levels of these key molecules may be important for t...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697657/ https://www.ncbi.nlm.nih.gov/pubmed/33202998 http://dx.doi.org/10.3390/diagnostics10110949 |
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author | Sato, Younosuke Okamoto, Isamu Kameyama, Hiroki Kudoh, Shinji Saito, Haruki Sanada, Mune Kudo, Noritaka Wakimoto, Joeji Fujino, Kosuke Ikematsu, Yuki Tanaka, Kentaro Nishikawa, Ayako Sakaguchi, Ryo Ito, Takaaki |
author_facet | Sato, Younosuke Okamoto, Isamu Kameyama, Hiroki Kudoh, Shinji Saito, Haruki Sanada, Mune Kudo, Noritaka Wakimoto, Joeji Fujino, Kosuke Ikematsu, Yuki Tanaka, Kentaro Nishikawa, Ayako Sakaguchi, Ryo Ito, Takaaki |
author_sort | Sato, Younosuke |
collection | PubMed |
description | Small-cell lung cancer (SCLC) is an aggressive malignant cancer that is classified into four subtypes based on the expression of the following key transcription and co-transcription factors: ASCL1, NEUROD1, YAP1, and POU2F3. The protein expression levels of these key molecules may be important for the formation of SCLC characteristics in a molecular subtype-specific manner. We expect that immunohistochemistry (IHC) of these molecules may facilitate the diagnosis of the specific SCLC molecular subtype and aid in the appropriate selection of individualized treatments. We attempted IHC of the four key factors and 26 candidate SCLC target molecules selected from the gene expression omnibus datasets of 47 SCLC samples, which were grouped based on positive or negative results for the four key molecules. We examined differences in the expression levels of the candidate targets and key molecules. ASCL1 showed the highest positive rate in SCLC samples, and significant differences were observed in the expression levels of some target molecules between the ASCL1-positive and ASCL1-negative groups. Furthermore, the four key molecules were coordinately and simultaneously expressed in SCLC cells. An IHC study of ASCL1-positive samples showed many candidate SCLC target molecules, and IHC could become an essential method for determining SCLC molecular subtypes. |
format | Online Article Text |
id | pubmed-7697657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76976572020-11-29 Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors Sato, Younosuke Okamoto, Isamu Kameyama, Hiroki Kudoh, Shinji Saito, Haruki Sanada, Mune Kudo, Noritaka Wakimoto, Joeji Fujino, Kosuke Ikematsu, Yuki Tanaka, Kentaro Nishikawa, Ayako Sakaguchi, Ryo Ito, Takaaki Diagnostics (Basel) Article Small-cell lung cancer (SCLC) is an aggressive malignant cancer that is classified into four subtypes based on the expression of the following key transcription and co-transcription factors: ASCL1, NEUROD1, YAP1, and POU2F3. The protein expression levels of these key molecules may be important for the formation of SCLC characteristics in a molecular subtype-specific manner. We expect that immunohistochemistry (IHC) of these molecules may facilitate the diagnosis of the specific SCLC molecular subtype and aid in the appropriate selection of individualized treatments. We attempted IHC of the four key factors and 26 candidate SCLC target molecules selected from the gene expression omnibus datasets of 47 SCLC samples, which were grouped based on positive or negative results for the four key molecules. We examined differences in the expression levels of the candidate targets and key molecules. ASCL1 showed the highest positive rate in SCLC samples, and significant differences were observed in the expression levels of some target molecules between the ASCL1-positive and ASCL1-negative groups. Furthermore, the four key molecules were coordinately and simultaneously expressed in SCLC cells. An IHC study of ASCL1-positive samples showed many candidate SCLC target molecules, and IHC could become an essential method for determining SCLC molecular subtypes. MDPI 2020-11-13 /pmc/articles/PMC7697657/ /pubmed/33202998 http://dx.doi.org/10.3390/diagnostics10110949 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sato, Younosuke Okamoto, Isamu Kameyama, Hiroki Kudoh, Shinji Saito, Haruki Sanada, Mune Kudo, Noritaka Wakimoto, Joeji Fujino, Kosuke Ikematsu, Yuki Tanaka, Kentaro Nishikawa, Ayako Sakaguchi, Ryo Ito, Takaaki Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors |
title | Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors |
title_full | Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors |
title_fullStr | Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors |
title_full_unstemmed | Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors |
title_short | Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors |
title_sort | integrated immunohistochemical study on small-cell carcinoma of the lung focusing on transcription and co-transcription factors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697657/ https://www.ncbi.nlm.nih.gov/pubmed/33202998 http://dx.doi.org/10.3390/diagnostics10110949 |
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