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Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans

Dengue fever is a rapidly emerging vector-borne viral disease with a growing global burden of approximately 390 million new infections per annum. The Dengue virus (DENV) is a flavivirus spread by female mosquitos of the aedes genus, but the mechanism of viral endocytosis is poorly understood at a mo...

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Autores principales: Hyatt, James G., Prévost, Sylvain, Devos, Juliette M., Mycroft-West, Courtney J., Skidmore, Mark A., Winter, Anja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697694/
https://www.ncbi.nlm.nih.gov/pubmed/33187224
http://dx.doi.org/10.3390/pathogens9110935
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author Hyatt, James G.
Prévost, Sylvain
Devos, Juliette M.
Mycroft-West, Courtney J.
Skidmore, Mark A.
Winter, Anja
author_facet Hyatt, James G.
Prévost, Sylvain
Devos, Juliette M.
Mycroft-West, Courtney J.
Skidmore, Mark A.
Winter, Anja
author_sort Hyatt, James G.
collection PubMed
description Dengue fever is a rapidly emerging vector-borne viral disease with a growing global burden of approximately 390 million new infections per annum. The Dengue virus (DENV) is a flavivirus spread by female mosquitos of the aedes genus, but the mechanism of viral endocytosis is poorly understood at a molecular level, preventing the development of effective transmission blocking vaccines (TBVs). Recently, glycosaminoglycans (GAGs) have been identified as playing a role during initial viral attachment through interaction with the third domain of the viral envelope protein (EDIII). Here, we report a systematic study investigating the effect of a range of biologically relevant GAGs on the structure and oligomeric state of recombinantly generated EDIII. We provide novel in situ biophysical evidence that heparin and chondroitin sulphate C induce conformational changes in EDIII at the secondary structure level. Furthermore, we report the ability of chondroitin sulphate C to bind EDIII and induce higher-order dynamic molecular changes at the tertiary and quaternary structure levels which are dependent on pH, GAG species, and the GAG sulphation state. Lastly, we conducted ab initio modelling of Small Angle Neutron Scattering (SANS) data to visualise the induced oligomeric state of EDIII caused by interaction with chondroitin sulphate C, which may aid in TBV development.
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spelling pubmed-76976942020-11-29 Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans Hyatt, James G. Prévost, Sylvain Devos, Juliette M. Mycroft-West, Courtney J. Skidmore, Mark A. Winter, Anja Pathogens Article Dengue fever is a rapidly emerging vector-borne viral disease with a growing global burden of approximately 390 million new infections per annum. The Dengue virus (DENV) is a flavivirus spread by female mosquitos of the aedes genus, but the mechanism of viral endocytosis is poorly understood at a molecular level, preventing the development of effective transmission blocking vaccines (TBVs). Recently, glycosaminoglycans (GAGs) have been identified as playing a role during initial viral attachment through interaction with the third domain of the viral envelope protein (EDIII). Here, we report a systematic study investigating the effect of a range of biologically relevant GAGs on the structure and oligomeric state of recombinantly generated EDIII. We provide novel in situ biophysical evidence that heparin and chondroitin sulphate C induce conformational changes in EDIII at the secondary structure level. Furthermore, we report the ability of chondroitin sulphate C to bind EDIII and induce higher-order dynamic molecular changes at the tertiary and quaternary structure levels which are dependent on pH, GAG species, and the GAG sulphation state. Lastly, we conducted ab initio modelling of Small Angle Neutron Scattering (SANS) data to visualise the induced oligomeric state of EDIII caused by interaction with chondroitin sulphate C, which may aid in TBV development. MDPI 2020-11-11 /pmc/articles/PMC7697694/ /pubmed/33187224 http://dx.doi.org/10.3390/pathogens9110935 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hyatt, James G.
Prévost, Sylvain
Devos, Juliette M.
Mycroft-West, Courtney J.
Skidmore, Mark A.
Winter, Anja
Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans
title Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans
title_full Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans
title_fullStr Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans
title_full_unstemmed Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans
title_short Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans
title_sort molecular changes in dengue envelope protein domain iii upon interaction with glycosaminoglycans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697694/
https://www.ncbi.nlm.nih.gov/pubmed/33187224
http://dx.doi.org/10.3390/pathogens9110935
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