Cargando…

Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces

Ultraviolet (UV) light and non-thermal plasma (NTP) are promising chair-side surface treatment methods to overcome the time-dependent aging of dental implant surfaces. After showing the efficiency of UV light and NTP treatment in restoring the biological activity of titanium and zirconia surfaces in...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Linna, Zou, Ziang, Smeets, Ralf, Kluwe, Lan, Hartjen, Philip, Cacaci, Claudio, Gosau, Martin, Henningsen, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697706/
https://www.ncbi.nlm.nih.gov/pubmed/33202662
http://dx.doi.org/10.3390/ijms21228598
_version_ 1783615659457904640
author Guo, Linna
Zou, Ziang
Smeets, Ralf
Kluwe, Lan
Hartjen, Philip
Cacaci, Claudio
Gosau, Martin
Henningsen, Anders
author_facet Guo, Linna
Zou, Ziang
Smeets, Ralf
Kluwe, Lan
Hartjen, Philip
Cacaci, Claudio
Gosau, Martin
Henningsen, Anders
author_sort Guo, Linna
collection PubMed
description Ultraviolet (UV) light and non-thermal plasma (NTP) are promising chair-side surface treatment methods to overcome the time-dependent aging of dental implant surfaces. After showing the efficiency of UV light and NTP treatment in restoring the biological activity of titanium and zirconia surfaces in vitro, the objective of this study was to define appropriate processing times for clinical use. Titanium and zirconia disks were treated by UV light and non-thermal oxygen plasma with increasing duration. Non-treated disks were set as controls. Murine osteoblast-like cells (MC3T3-E1) were seeded onto the treated or non-treated disks. After 2 and 24 h of incubation, the viability of cells on surfaces was assessed using an MTS assay. mRNA expression of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were assessed using real-time reverse transcription polymerase chain reaction analysis. Cellular morphology and attachment were observed using confocal microscopy. The viability of MC3T3-E1 was significantly increased in 12 min UV-light treated and 1 min oxygen NTP treated groups. VEGF relative expression reached the highest levels on 12 min UV-light and 1 min NTP treated surfaces of both disks. The highest levels of HGF relative expression were reached on 12 min UV light treated zirconia surfaces. However, cells on 12 and 16 min UV-light and NTP treated surfaces of both materials had a more widely spread cytoskeleton compared to control groups. Twelve min UV-light and one min non-thermal oxygen plasma treatment on titanium and zirconia may be the favored times in terms of increasing the viability, mRNA expression of growth factors and cellular attachment in MC3T3-E1 cells.
format Online
Article
Text
id pubmed-7697706
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76977062020-11-29 Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces Guo, Linna Zou, Ziang Smeets, Ralf Kluwe, Lan Hartjen, Philip Cacaci, Claudio Gosau, Martin Henningsen, Anders Int J Mol Sci Article Ultraviolet (UV) light and non-thermal plasma (NTP) are promising chair-side surface treatment methods to overcome the time-dependent aging of dental implant surfaces. After showing the efficiency of UV light and NTP treatment in restoring the biological activity of titanium and zirconia surfaces in vitro, the objective of this study was to define appropriate processing times for clinical use. Titanium and zirconia disks were treated by UV light and non-thermal oxygen plasma with increasing duration. Non-treated disks were set as controls. Murine osteoblast-like cells (MC3T3-E1) were seeded onto the treated or non-treated disks. After 2 and 24 h of incubation, the viability of cells on surfaces was assessed using an MTS assay. mRNA expression of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were assessed using real-time reverse transcription polymerase chain reaction analysis. Cellular morphology and attachment were observed using confocal microscopy. The viability of MC3T3-E1 was significantly increased in 12 min UV-light treated and 1 min oxygen NTP treated groups. VEGF relative expression reached the highest levels on 12 min UV-light and 1 min NTP treated surfaces of both disks. The highest levels of HGF relative expression were reached on 12 min UV light treated zirconia surfaces. However, cells on 12 and 16 min UV-light and NTP treated surfaces of both materials had a more widely spread cytoskeleton compared to control groups. Twelve min UV-light and one min non-thermal oxygen plasma treatment on titanium and zirconia may be the favored times in terms of increasing the viability, mRNA expression of growth factors and cellular attachment in MC3T3-E1 cells. MDPI 2020-11-14 /pmc/articles/PMC7697706/ /pubmed/33202662 http://dx.doi.org/10.3390/ijms21228598 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Linna
Zou, Ziang
Smeets, Ralf
Kluwe, Lan
Hartjen, Philip
Cacaci, Claudio
Gosau, Martin
Henningsen, Anders
Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces
title Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces
title_full Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces
title_fullStr Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces
title_full_unstemmed Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces
title_short Time Dependency of Non-Thermal Oxygen Plasma and Ultraviolet Irradiation on Cellular Attachment and mRNA Expression of Growth Factors in Osteoblasts on Titanium and Zirconia Surfaces
title_sort time dependency of non-thermal oxygen plasma and ultraviolet irradiation on cellular attachment and mrna expression of growth factors in osteoblasts on titanium and zirconia surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697706/
https://www.ncbi.nlm.nih.gov/pubmed/33202662
http://dx.doi.org/10.3390/ijms21228598
work_keys_str_mv AT guolinna timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT zouziang timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT smeetsralf timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT kluwelan timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT hartjenphilip timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT cacaciclaudio timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT gosaumartin timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces
AT henningsenanders timedependencyofnonthermaloxygenplasmaandultravioletirradiationoncellularattachmentandmrnaexpressionofgrowthfactorsinosteoblastsontitaniumandzirconiasurfaces