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Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment

Dengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is pal...

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Autores principales: Ferreira, Jorge G. G., Gava, Sandra G., Oliveira, Eneida S., Batista, Izabella C. A., Fernandes, Gabriel da R., Mourão, Marina M., Calzavara-Silva, Carlos E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697769/
https://www.ncbi.nlm.nih.gov/pubmed/33182673
http://dx.doi.org/10.3390/v12111284
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author Ferreira, Jorge G. G.
Gava, Sandra G.
Oliveira, Eneida S.
Batista, Izabella C. A.
Fernandes, Gabriel da R.
Mourão, Marina M.
Calzavara-Silva, Carlos E.
author_facet Ferreira, Jorge G. G.
Gava, Sandra G.
Oliveira, Eneida S.
Batista, Izabella C. A.
Fernandes, Gabriel da R.
Mourão, Marina M.
Calzavara-Silva, Carlos E.
author_sort Ferreira, Jorge G. G.
collection PubMed
description Dengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus–drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis.
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spelling pubmed-76977692020-11-29 Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment Ferreira, Jorge G. G. Gava, Sandra G. Oliveira, Eneida S. Batista, Izabella C. A. Fernandes, Gabriel da R. Mourão, Marina M. Calzavara-Silva, Carlos E. Viruses Article Dengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus–drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis. MDPI 2020-11-10 /pmc/articles/PMC7697769/ /pubmed/33182673 http://dx.doi.org/10.3390/v12111284 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferreira, Jorge G. G.
Gava, Sandra G.
Oliveira, Eneida S.
Batista, Izabella C. A.
Fernandes, Gabriel da R.
Mourão, Marina M.
Calzavara-Silva, Carlos E.
Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_full Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_fullStr Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_full_unstemmed Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_short Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
title_sort gene expression signatures in aml-12 hepatocyte cells upon dengue virus infection and acetaminophen treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697769/
https://www.ncbi.nlm.nih.gov/pubmed/33182673
http://dx.doi.org/10.3390/v12111284
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