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Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System

The petals of the saffron crocus (Crocus sativus L.) are considered a waste material in saffron production, but may be a sustainable source of natural biologically active substances of nutraceutical interest. The aim of this work was to study the cardiovascular effects of kaempferol and crocin extra...

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Autores principales: Zeka, Keti, Marrazzo, Pasquale, Micucci, Matteo, Ruparelia, Ketan C., Arroo, Randolph R. J., Macchiarelli, Guido, Annarita Nottola, Stefania, Continenza, Maria Adelaide, Chiarini, Alberto, Angeloni, Cristina, Hrelia, Silvana, Budriesi, Roberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697793/
https://www.ncbi.nlm.nih.gov/pubmed/33182461
http://dx.doi.org/10.3390/antiox9111102
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author Zeka, Keti
Marrazzo, Pasquale
Micucci, Matteo
Ruparelia, Ketan C.
Arroo, Randolph R. J.
Macchiarelli, Guido
Annarita Nottola, Stefania
Continenza, Maria Adelaide
Chiarini, Alberto
Angeloni, Cristina
Hrelia, Silvana
Budriesi, Roberta
author_facet Zeka, Keti
Marrazzo, Pasquale
Micucci, Matteo
Ruparelia, Ketan C.
Arroo, Randolph R. J.
Macchiarelli, Guido
Annarita Nottola, Stefania
Continenza, Maria Adelaide
Chiarini, Alberto
Angeloni, Cristina
Hrelia, Silvana
Budriesi, Roberta
author_sort Zeka, Keti
collection PubMed
description The petals of the saffron crocus (Crocus sativus L.) are considered a waste material in saffron production, but may be a sustainable source of natural biologically active substances of nutraceutical interest. The aim of this work was to study the cardiovascular effects of kaempferol and crocin extracted from saffron petals. The antiarrhythmic, inotropic, and chronotropic effects of saffron petal extract (SPE), kaempferol, and crocin were evaluated through in vitro biological assays. The antioxidant activity of kaempferol and crocin was investigated through the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay using rat cardiomyoblast cell line H9c2. The MTT assay was applied to assess the effects of kaempferol and crocin on cell viability. SPE showed weak negative inotropic and chronotropic intrinsic activities but a significant intrinsic activity on smooth muscle with a potency on the ileum greater than on the aorta: EC(50) = 0.66 mg/mL versus EC(50) = 1.45 mg/mL. Kaempferol and crocin showed a selective negative inotropic activity. In addition, kaempferol decreased the contraction induced by KCl (80 mM) in guinea pig aortic and ileal strips, while crocin had no effect. Furthermore, following oxidative stress, both crocin and kaempferol decreased intracellular ROS formation and increased cell viability in a concentration-dependent manner. The results indicate that SPE, a by-product of saffron cultivation, may represent a good source of phytochemicals with a potential application in the prevention of cardiovascular diseases.
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spelling pubmed-76977932020-11-29 Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System Zeka, Keti Marrazzo, Pasquale Micucci, Matteo Ruparelia, Ketan C. Arroo, Randolph R. J. Macchiarelli, Guido Annarita Nottola, Stefania Continenza, Maria Adelaide Chiarini, Alberto Angeloni, Cristina Hrelia, Silvana Budriesi, Roberta Antioxidants (Basel) Article The petals of the saffron crocus (Crocus sativus L.) are considered a waste material in saffron production, but may be a sustainable source of natural biologically active substances of nutraceutical interest. The aim of this work was to study the cardiovascular effects of kaempferol and crocin extracted from saffron petals. The antiarrhythmic, inotropic, and chronotropic effects of saffron petal extract (SPE), kaempferol, and crocin were evaluated through in vitro biological assays. The antioxidant activity of kaempferol and crocin was investigated through the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay using rat cardiomyoblast cell line H9c2. The MTT assay was applied to assess the effects of kaempferol and crocin on cell viability. SPE showed weak negative inotropic and chronotropic intrinsic activities but a significant intrinsic activity on smooth muscle with a potency on the ileum greater than on the aorta: EC(50) = 0.66 mg/mL versus EC(50) = 1.45 mg/mL. Kaempferol and crocin showed a selective negative inotropic activity. In addition, kaempferol decreased the contraction induced by KCl (80 mM) in guinea pig aortic and ileal strips, while crocin had no effect. Furthermore, following oxidative stress, both crocin and kaempferol decreased intracellular ROS formation and increased cell viability in a concentration-dependent manner. The results indicate that SPE, a by-product of saffron cultivation, may represent a good source of phytochemicals with a potential application in the prevention of cardiovascular diseases. MDPI 2020-11-09 /pmc/articles/PMC7697793/ /pubmed/33182461 http://dx.doi.org/10.3390/antiox9111102 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zeka, Keti
Marrazzo, Pasquale
Micucci, Matteo
Ruparelia, Ketan C.
Arroo, Randolph R. J.
Macchiarelli, Guido
Annarita Nottola, Stefania
Continenza, Maria Adelaide
Chiarini, Alberto
Angeloni, Cristina
Hrelia, Silvana
Budriesi, Roberta
Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
title Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
title_full Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
title_fullStr Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
title_full_unstemmed Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
title_short Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
title_sort activity of antioxidants from crocus sativus l. petals: potential preventive effects towards cardiovascular system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697793/
https://www.ncbi.nlm.nih.gov/pubmed/33182461
http://dx.doi.org/10.3390/antiox9111102
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