Cargando…
First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora
Newly discovered Erwinia amylovora phages PEar1, PEar2, PEar4 and PEar6 were isolated from three different orchards in North Tunisia to study their potential as biocontrol agents. Illumina sequencing revealed that the PEar viruses carry a single-strand DNA genome between 6608 and 6801 nucleotides an...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697814/ https://www.ncbi.nlm.nih.gov/pubmed/33182526 http://dx.doi.org/10.3390/microorganisms8111762 |
| _version_ | 1783615684848123904 |
|---|---|
| author | Akremi, Ismahen Holtappels, Dominique Brabra, Wided Jlidi, Mouna Hadj Ibrahim, Adel Ben Ali, Manel Fortuna, Kiandro Ahmed, Mohammed Meerbeek, Bart Van Rhouma, Ali Lavigne, Rob Ben Ali, Mamdouh Wagemans, Jeroen |
| author_facet | Akremi, Ismahen Holtappels, Dominique Brabra, Wided Jlidi, Mouna Hadj Ibrahim, Adel Ben Ali, Manel Fortuna, Kiandro Ahmed, Mohammed Meerbeek, Bart Van Rhouma, Ali Lavigne, Rob Ben Ali, Mamdouh Wagemans, Jeroen |
| author_sort | Akremi, Ismahen |
| collection | PubMed |
| description | Newly discovered Erwinia amylovora phages PEar1, PEar2, PEar4 and PEar6 were isolated from three different orchards in North Tunisia to study their potential as biocontrol agents. Illumina sequencing revealed that the PEar viruses carry a single-strand DNA genome between 6608 and 6801 nucleotides and belong to the Inoviridae, making them the first described filamentous phages of E. amylovora. Interestingly, phage-infected cells show a decreased swimming and swarming motility and a cocktail of the four phages can significantly reduce infection of E. amylovora in a pear bioassay, potentially making them suitable candidates for phage biocontrol. |
| format | Online Article Text |
| id | pubmed-7697814 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-76978142020-11-29 First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora Akremi, Ismahen Holtappels, Dominique Brabra, Wided Jlidi, Mouna Hadj Ibrahim, Adel Ben Ali, Manel Fortuna, Kiandro Ahmed, Mohammed Meerbeek, Bart Van Rhouma, Ali Lavigne, Rob Ben Ali, Mamdouh Wagemans, Jeroen Microorganisms Article Newly discovered Erwinia amylovora phages PEar1, PEar2, PEar4 and PEar6 were isolated from three different orchards in North Tunisia to study their potential as biocontrol agents. Illumina sequencing revealed that the PEar viruses carry a single-strand DNA genome between 6608 and 6801 nucleotides and belong to the Inoviridae, making them the first described filamentous phages of E. amylovora. Interestingly, phage-infected cells show a decreased swimming and swarming motility and a cocktail of the four phages can significantly reduce infection of E. amylovora in a pear bioassay, potentially making them suitable candidates for phage biocontrol. MDPI 2020-11-10 /pmc/articles/PMC7697814/ /pubmed/33182526 http://dx.doi.org/10.3390/microorganisms8111762 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Akremi, Ismahen Holtappels, Dominique Brabra, Wided Jlidi, Mouna Hadj Ibrahim, Adel Ben Ali, Manel Fortuna, Kiandro Ahmed, Mohammed Meerbeek, Bart Van Rhouma, Ali Lavigne, Rob Ben Ali, Mamdouh Wagemans, Jeroen First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora |
| title | First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora |
| title_full | First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora |
| title_fullStr | First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora |
| title_full_unstemmed | First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora |
| title_short | First Report of Filamentous Phages Isolated from Tunisian Orchards to Control Erwinia amylovora |
| title_sort | first report of filamentous phages isolated from tunisian orchards to control erwinia amylovora |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697814/ https://www.ncbi.nlm.nih.gov/pubmed/33182526 http://dx.doi.org/10.3390/microorganisms8111762 |
| work_keys_str_mv | AT akremiismahen firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT holtappelsdominique firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT brabrawided firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT jlidimouna firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT hadjibrahimadel firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT benalimanel firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT fortunakiandro firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT ahmedmohammed firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT meerbeekbartvan firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT rhoumaali firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT lavignerob firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT benalimamdouh firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora AT wagemansjeroen firstreportoffilamentousphagesisolatedfromtunisianorchardstocontrolerwiniaamylovora |