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Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence

The present work describes synthesis, characterization, and use of a new dansyl-labelled Ag@SiO(2) nanocomposite as an element of a new plasmonic platform to enhance the fluorescence intensity. Keeping in mind that typical surface plasmon resonance (SPR) characteristics of silver nanoparticles coinc...

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Autores principales: Szczepańska, Elżbieta, Synak, Anna, Bojarski, Piotr, Niedziałkowski, Paweł, Wcisło, Anna, Ossowski, Tadeusz, Grobelna, Beata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697960/
https://www.ncbi.nlm.nih.gov/pubmed/33207805
http://dx.doi.org/10.3390/ma13225168
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author Szczepańska, Elżbieta
Synak, Anna
Bojarski, Piotr
Niedziałkowski, Paweł
Wcisło, Anna
Ossowski, Tadeusz
Grobelna, Beata
author_facet Szczepańska, Elżbieta
Synak, Anna
Bojarski, Piotr
Niedziałkowski, Paweł
Wcisło, Anna
Ossowski, Tadeusz
Grobelna, Beata
author_sort Szczepańska, Elżbieta
collection PubMed
description The present work describes synthesis, characterization, and use of a new dansyl-labelled Ag@SiO(2) nanocomposite as an element of a new plasmonic platform to enhance the fluorescence intensity. Keeping in mind that typical surface plasmon resonance (SPR) characteristics of silver nanoparticles coincide well enough with the absorption of dansyl molecules, we used them to build the core of the nanocomposite. Moreover, we utilized 10 nm amino-functionalized silica shell as a separator between silver nanoparticles and the dansyl dye to prevent the dye-to-metal energy transfer. The dansyl group was incorporated into Ag@SiO(2) core-shell nanostructures by the reaction of aminopropyltrimethoxysilane with dansyl chloride and we characterized the new dansyl-labelled Ag@SiO(2) nanocomposite using transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Additionally, water wettability measurements (WWM) were carried out to assess the hydrophobicity and hydrophilicity of the studied surface. We found that the nanocomposite deposited on a semitransparent silver mirror strongly increased the fluorescence intensity of dansyl dye (about 87-fold) compared with the control sample on the glass, proving that the system is a perfect candidate for a sensitive plasmonic platform.
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spelling pubmed-76979602020-11-29 Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence Szczepańska, Elżbieta Synak, Anna Bojarski, Piotr Niedziałkowski, Paweł Wcisło, Anna Ossowski, Tadeusz Grobelna, Beata Materials (Basel) Article The present work describes synthesis, characterization, and use of a new dansyl-labelled Ag@SiO(2) nanocomposite as an element of a new plasmonic platform to enhance the fluorescence intensity. Keeping in mind that typical surface plasmon resonance (SPR) characteristics of silver nanoparticles coincide well enough with the absorption of dansyl molecules, we used them to build the core of the nanocomposite. Moreover, we utilized 10 nm amino-functionalized silica shell as a separator between silver nanoparticles and the dansyl dye to prevent the dye-to-metal energy transfer. The dansyl group was incorporated into Ag@SiO(2) core-shell nanostructures by the reaction of aminopropyltrimethoxysilane with dansyl chloride and we characterized the new dansyl-labelled Ag@SiO(2) nanocomposite using transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Additionally, water wettability measurements (WWM) were carried out to assess the hydrophobicity and hydrophilicity of the studied surface. We found that the nanocomposite deposited on a semitransparent silver mirror strongly increased the fluorescence intensity of dansyl dye (about 87-fold) compared with the control sample on the glass, proving that the system is a perfect candidate for a sensitive plasmonic platform. MDPI 2020-11-16 /pmc/articles/PMC7697960/ /pubmed/33207805 http://dx.doi.org/10.3390/ma13225168 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szczepańska, Elżbieta
Synak, Anna
Bojarski, Piotr
Niedziałkowski, Paweł
Wcisło, Anna
Ossowski, Tadeusz
Grobelna, Beata
Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence
title Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence
title_full Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence
title_fullStr Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence
title_full_unstemmed Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence
title_short Dansyl-Labelled Ag@SiO(2) Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence
title_sort dansyl-labelled ag@sio(2) core-shell nanostructures—synthesis, characterization, and metal-enhanced fluorescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697960/
https://www.ncbi.nlm.nih.gov/pubmed/33207805
http://dx.doi.org/10.3390/ma13225168
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