Cargando…
Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes
SIMPLE SUMMARY: Previous studies revealed that alternative splicing (AS) events and gene variants played key roles in reproduction. However, their location and distribution in hypothalamic fecundity-related genes in sheep without the FecB mutation remain largely unknown. In this study, we performed...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698220/ https://www.ncbi.nlm.nih.gov/pubmed/33203033 http://dx.doi.org/10.3390/ani10112111 |
_version_ | 1783615780075601920 |
---|---|
author | Zhang, Zhuangbiao Tang, Jishun He, Xiaoyun Di, Ran Zhang, Xiaosheng Zhang, Jinlong Hu, Wenping Chu, Mingxing |
author_facet | Zhang, Zhuangbiao Tang, Jishun He, Xiaoyun Di, Ran Zhang, Xiaosheng Zhang, Jinlong Hu, Wenping Chu, Mingxing |
author_sort | Zhang, Zhuangbiao |
collection | PubMed |
description | SIMPLE SUMMARY: Previous studies revealed that alternative splicing (AS) events and gene variants played key roles in reproduction. However, their location and distribution in hypothalamic fecundity-related genes in sheep without the FecB mutation remain largely unknown. In this study, we performed a correlation analysis of transcriptomics and proteomics, and the results suggested several differentially expressed genes (DEGs)/differentially expressed proteins (DEPs), including galectin 3 (LGALS3), aspartoacylase (ASPA) and transthyretin (TTR), could be candidate genes influencing ovine litter size. Further analysis suggested that AS events, single nucleotide polymorphisms (SNPs) and microRNA (miRNA)-binding sites existed in key DEGs/DEPs, such as ASPA and TTR. This study provides a new insight into ovine and even other mammalian reproduction. ABSTRACT: Previous studies revealed that alternative splicing (AS) events and gene variants played key roles in reproduction; however, their location and distribution in hypothalamic fecundity-related genes in sheep without the FecB mutation remain largely unknown. Therefore, in this study, we described the hypothalamic AS events and variants in differentially expressed genes (DEGs) in Small Tail Han sheep without the FecB mutation at polytocous sheep in the follicular phase vs. monotocous sheep in the follicular phase (PF vs. MF) and polytocous sheep in the luteal phase vs. monotocous sheep in the luteal phase (PL vs. ML) via an RNA-seq study for the first time. We found 39 DEGs with AS events (AS DEGs) in PF vs. MF, while 42 AS DEGs were identified in PL vs. ML. No DEGs with single nucleotide polymorphisms (SNPs) were observed in PF vs. MF, but five were identified in PL vs. ML. We also performed a correlation analysis of transcriptomics and proteomics, and the results suggested several key DEGs/differentially expressed proteins (DEPs), such as galectin 3 (LGALS3) in PF vs. MF and aspartoacylase (ASPA) and transthyretin (TTR) in PL vs. ML, could be candidate genes influencing ovine litter size. In addition, further analyses suggested that AS events, SNPs and miRNA-binding sites existed in key DEGs/DEPs, such as ASPA and TTR. All in all, this study provides a new insight into ovine and even other mammalian reproduction. |
format | Online Article Text |
id | pubmed-7698220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76982202020-11-29 Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes Zhang, Zhuangbiao Tang, Jishun He, Xiaoyun Di, Ran Zhang, Xiaosheng Zhang, Jinlong Hu, Wenping Chu, Mingxing Animals (Basel) Article SIMPLE SUMMARY: Previous studies revealed that alternative splicing (AS) events and gene variants played key roles in reproduction. However, their location and distribution in hypothalamic fecundity-related genes in sheep without the FecB mutation remain largely unknown. In this study, we performed a correlation analysis of transcriptomics and proteomics, and the results suggested several differentially expressed genes (DEGs)/differentially expressed proteins (DEPs), including galectin 3 (LGALS3), aspartoacylase (ASPA) and transthyretin (TTR), could be candidate genes influencing ovine litter size. Further analysis suggested that AS events, single nucleotide polymorphisms (SNPs) and microRNA (miRNA)-binding sites existed in key DEGs/DEPs, such as ASPA and TTR. This study provides a new insight into ovine and even other mammalian reproduction. ABSTRACT: Previous studies revealed that alternative splicing (AS) events and gene variants played key roles in reproduction; however, their location and distribution in hypothalamic fecundity-related genes in sheep without the FecB mutation remain largely unknown. Therefore, in this study, we described the hypothalamic AS events and variants in differentially expressed genes (DEGs) in Small Tail Han sheep without the FecB mutation at polytocous sheep in the follicular phase vs. monotocous sheep in the follicular phase (PF vs. MF) and polytocous sheep in the luteal phase vs. monotocous sheep in the luteal phase (PL vs. ML) via an RNA-seq study for the first time. We found 39 DEGs with AS events (AS DEGs) in PF vs. MF, while 42 AS DEGs were identified in PL vs. ML. No DEGs with single nucleotide polymorphisms (SNPs) were observed in PF vs. MF, but five were identified in PL vs. ML. We also performed a correlation analysis of transcriptomics and proteomics, and the results suggested several key DEGs/differentially expressed proteins (DEPs), such as galectin 3 (LGALS3) in PF vs. MF and aspartoacylase (ASPA) and transthyretin (TTR) in PL vs. ML, could be candidate genes influencing ovine litter size. In addition, further analyses suggested that AS events, SNPs and miRNA-binding sites existed in key DEGs/DEPs, such as ASPA and TTR. All in all, this study provides a new insight into ovine and even other mammalian reproduction. MDPI 2020-11-13 /pmc/articles/PMC7698220/ /pubmed/33203033 http://dx.doi.org/10.3390/ani10112111 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Zhuangbiao Tang, Jishun He, Xiaoyun Di, Ran Zhang, Xiaosheng Zhang, Jinlong Hu, Wenping Chu, Mingxing Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes |
title | Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes |
title_full | Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes |
title_fullStr | Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes |
title_full_unstemmed | Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes |
title_short | Identification and Characterization of Hypothalamic Alternative Splicing Events and Variants in Ovine Fecundity-Related Genes |
title_sort | identification and characterization of hypothalamic alternative splicing events and variants in ovine fecundity-related genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698220/ https://www.ncbi.nlm.nih.gov/pubmed/33203033 http://dx.doi.org/10.3390/ani10112111 |
work_keys_str_mv | AT zhangzhuangbiao identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT tangjishun identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT hexiaoyun identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT diran identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT zhangxiaosheng identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT zhangjinlong identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT huwenping identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes AT chumingxing identificationandcharacterizationofhypothalamicalternativesplicingeventsandvariantsinovinefecundityrelatedgenes |