Cargando…
Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer
Fluorine-19 MRI is a promising noninvasive diagnostic method. However, the absence of a nontoxic fluorine-19 MRI tracer that does not suffer from poor biodistribution as a result of its strong fluorophilicity is a constant hurdle in the widespread applicability of this otherwise versatile diagnostic...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698257/ https://www.ncbi.nlm.nih.gov/pubmed/33182714 http://dx.doi.org/10.3390/nano10112231 |
_version_ | 1783615788736839680 |
---|---|
author | Babuka, David Kolouchova, Kristyna Groborz, Ondrej Tosner, Zdenek Zhigunov, Alexander Stepanek, Petr Hruby, Martin |
author_facet | Babuka, David Kolouchova, Kristyna Groborz, Ondrej Tosner, Zdenek Zhigunov, Alexander Stepanek, Petr Hruby, Martin |
author_sort | Babuka, David |
collection | PubMed |
description | Fluorine-19 MRI is a promising noninvasive diagnostic method. However, the absence of a nontoxic fluorine-19 MRI tracer that does not suffer from poor biodistribution as a result of its strong fluorophilicity is a constant hurdle in the widespread applicability of this otherwise versatile diagnostic technique. The poly[N-(2-hydroxypropyl)methacrylamide]-block-poly[N-(2,2-difluoroethyl)acrylamide] thermoresponsive copolymer was proposed as an alternative fluorine-19 MRI tracer capable of overcoming such shortcomings. In this paper, the internal structure of self-assembled particles of this copolymer was investigated by various methods including 1D and 2D NMR, dynamic light scattering (DLS), small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS). The elucidated structure appears to be that of a nanogel with greatly swollen hydrophilic chains and tightly packed thermoresponsive chains forming a network within the nanogel particles, which become more hydrophobic with increasing temperature. Its capacity to provide a measurable fluorine-19 NMR signal in its aggregated state at human body temperature was also investigated and confirmed. This capacity stems from the different fluorine-19 nuclei relaxation properties compared to those of hydrogen-1 nuclei. |
format | Online Article Text |
id | pubmed-7698257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76982572020-11-29 Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer Babuka, David Kolouchova, Kristyna Groborz, Ondrej Tosner, Zdenek Zhigunov, Alexander Stepanek, Petr Hruby, Martin Nanomaterials (Basel) Article Fluorine-19 MRI is a promising noninvasive diagnostic method. However, the absence of a nontoxic fluorine-19 MRI tracer that does not suffer from poor biodistribution as a result of its strong fluorophilicity is a constant hurdle in the widespread applicability of this otherwise versatile diagnostic technique. The poly[N-(2-hydroxypropyl)methacrylamide]-block-poly[N-(2,2-difluoroethyl)acrylamide] thermoresponsive copolymer was proposed as an alternative fluorine-19 MRI tracer capable of overcoming such shortcomings. In this paper, the internal structure of self-assembled particles of this copolymer was investigated by various methods including 1D and 2D NMR, dynamic light scattering (DLS), small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS). The elucidated structure appears to be that of a nanogel with greatly swollen hydrophilic chains and tightly packed thermoresponsive chains forming a network within the nanogel particles, which become more hydrophobic with increasing temperature. Its capacity to provide a measurable fluorine-19 NMR signal in its aggregated state at human body temperature was also investigated and confirmed. This capacity stems from the different fluorine-19 nuclei relaxation properties compared to those of hydrogen-1 nuclei. MDPI 2020-11-10 /pmc/articles/PMC7698257/ /pubmed/33182714 http://dx.doi.org/10.3390/nano10112231 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Babuka, David Kolouchova, Kristyna Groborz, Ondrej Tosner, Zdenek Zhigunov, Alexander Stepanek, Petr Hruby, Martin Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer |
title | Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer |
title_full | Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer |
title_fullStr | Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer |
title_full_unstemmed | Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer |
title_short | Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent (19)F MRI Tracer |
title_sort | internal structure of thermoresponsive physically crosslinked nanogel of poly[n-(2-hydroxypropyl)methacrylamide]-block-poly[n-(2,2-difluoroethyl)acrylamide], prominent (19)f mri tracer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698257/ https://www.ncbi.nlm.nih.gov/pubmed/33182714 http://dx.doi.org/10.3390/nano10112231 |
work_keys_str_mv | AT babukadavid internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer AT kolouchovakristyna internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer AT groborzondrej internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer AT tosnerzdenek internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer AT zhigunovalexander internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer AT stepanekpetr internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer AT hrubymartin internalstructureofthermoresponsivephysicallycrosslinkednanogelofpolyn2hydroxypropylmethacrylamideblockpolyn22difluoroethylacrylamideprominent19fmritracer |