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Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats

The effects of early-life fructose consumption on hepatic signaling pathways and their relation to the development of metabolic disorders in later life are not fully understood. To investigate whether fructose overconsumption at a young age induces alterations in glucocorticoid signaling that might...

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Autores principales: Elaković, Ivana, Kovačević, Sanja, Vojnović Milutinović, Danijela, Nikolić-Kokić, Aleksandra, Glban, Alhadi M., Spasić, Mihajlo, Tappy, Luc, Djordjevic, Ana, Matić, Gordana, Brkljačić, Jelena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698302/
https://www.ncbi.nlm.nih.gov/pubmed/33198224
http://dx.doi.org/10.3390/nu12113470
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author Elaković, Ivana
Kovačević, Sanja
Vojnović Milutinović, Danijela
Nikolić-Kokić, Aleksandra
Glban, Alhadi M.
Spasić, Mihajlo
Tappy, Luc
Djordjevic, Ana
Matić, Gordana
Brkljačić, Jelena
author_facet Elaković, Ivana
Kovačević, Sanja
Vojnović Milutinović, Danijela
Nikolić-Kokić, Aleksandra
Glban, Alhadi M.
Spasić, Mihajlo
Tappy, Luc
Djordjevic, Ana
Matić, Gordana
Brkljačić, Jelena
author_sort Elaković, Ivana
collection PubMed
description The effects of early-life fructose consumption on hepatic signaling pathways and their relation to the development of metabolic disorders in later life are not fully understood. To investigate whether fructose overconsumption at a young age induces alterations in glucocorticoid signaling that might contribute to development of metabolic disturbances, we analysed glucocorticoid receptor hormone-binding parameters and expression of its target genes involved in gluconeogenesis (phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) and lipid metabolism (lipin-1), as well as redox and inflammatory status in the liver of female rats subjected to a fructose-rich diet immediately after weaning. The fructose diet increased hepatic corticosterone concentration, 11β-hydroxysteroid dehydrogenase type 1 level, glucocorticoid receptor protein level and hormone-binding activity, as well as lipin-1 level. The expression of glucose-6-phosphatase was reduced in fructose-fed rats, while phosphoenolpyruvate carboxykinase remained unaltered. The fructose-rich diet increased the level of fructose transporter GLUT2, while the expression of fructolytic enzymes fructokinase and aldolase B remained unaltered. The diet also affected pro-inflammatory pathways, but had no effect on the antioxidant defence system. In conclusion, a fructose-rich diet applied immediately after weaning promoted lipogenesis and enhanced hepatic glucocorticoid signaling, possibly to protect against inflammatory damage, but without an effect on gluconeogenesis and antioxidant enzymes. Yet, prolonged treatment might ultimately lead to more pronounced metabolic disturbances.
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spelling pubmed-76983022020-11-29 Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats Elaković, Ivana Kovačević, Sanja Vojnović Milutinović, Danijela Nikolić-Kokić, Aleksandra Glban, Alhadi M. Spasić, Mihajlo Tappy, Luc Djordjevic, Ana Matić, Gordana Brkljačić, Jelena Nutrients Article The effects of early-life fructose consumption on hepatic signaling pathways and their relation to the development of metabolic disorders in later life are not fully understood. To investigate whether fructose overconsumption at a young age induces alterations in glucocorticoid signaling that might contribute to development of metabolic disturbances, we analysed glucocorticoid receptor hormone-binding parameters and expression of its target genes involved in gluconeogenesis (phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) and lipid metabolism (lipin-1), as well as redox and inflammatory status in the liver of female rats subjected to a fructose-rich diet immediately after weaning. The fructose diet increased hepatic corticosterone concentration, 11β-hydroxysteroid dehydrogenase type 1 level, glucocorticoid receptor protein level and hormone-binding activity, as well as lipin-1 level. The expression of glucose-6-phosphatase was reduced in fructose-fed rats, while phosphoenolpyruvate carboxykinase remained unaltered. The fructose-rich diet increased the level of fructose transporter GLUT2, while the expression of fructolytic enzymes fructokinase and aldolase B remained unaltered. The diet also affected pro-inflammatory pathways, but had no effect on the antioxidant defence system. In conclusion, a fructose-rich diet applied immediately after weaning promoted lipogenesis and enhanced hepatic glucocorticoid signaling, possibly to protect against inflammatory damage, but without an effect on gluconeogenesis and antioxidant enzymes. Yet, prolonged treatment might ultimately lead to more pronounced metabolic disturbances. MDPI 2020-11-12 /pmc/articles/PMC7698302/ /pubmed/33198224 http://dx.doi.org/10.3390/nu12113470 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elaković, Ivana
Kovačević, Sanja
Vojnović Milutinović, Danijela
Nikolić-Kokić, Aleksandra
Glban, Alhadi M.
Spasić, Mihajlo
Tappy, Luc
Djordjevic, Ana
Matić, Gordana
Brkljačić, Jelena
Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
title Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
title_full Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
title_fullStr Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
title_full_unstemmed Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
title_short Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
title_sort fructose consumption affects glucocorticoid signaling in the liver of young female rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698302/
https://www.ncbi.nlm.nih.gov/pubmed/33198224
http://dx.doi.org/10.3390/nu12113470
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