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Electrochemical Immunosensors Based on Screen-Printed Gold and Glassy Carbon Electrodes: Comparison of Performance for Respiratory Syncytial Virus Detection

This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was succes...

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Detalles Bibliográficos
Autores principales: Białobrzeska, Wioleta, Firganek, Daniel, Czerkies, Maciej, Lipniacki, Tomasz, Skwarecka, Marta, Dziąbowska, Karolina, Cebula, Zofia, Malinowska, Natalia, Bigus, Daniel, Bięga, Ewelina, Pyrć, Krzysztof, Pala, Katarzyna, Żołędowska, Sabina, Nidzworski, Dawid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698328/
https://www.ncbi.nlm.nih.gov/pubmed/33202922
http://dx.doi.org/10.3390/bios10110175
Descripción
Sumario:This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 10(5) PFU/mL to 1.5×10(7) PFU/mL, more than 1.0 × 10(5) PFU/mL to 1.0 × 10(7) PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 10(3) PFU/mL, three orders of magnitude lower than 2.85 × 10(6) PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.