Postweaning Grouping as a Strategy to Reduce Singly Housed Male Mice

SIMPLE SUMMARY: It is important to raise laboratory mice in stable groups. However, sometimes we can find litters with only one male. Unfortunately, age is a factor to consider when grouping a newly weaned mouse with other males due to inter-male aggressiveness. Our results showed that CD1 and SCID Beige newly weaned males can be safely grouped with juvenile and pubescent mice. However, only juvenile C57BL/6J will accept a newly weaned mouse as a new member of the group. This strategy could be helpful to reduce the number of singly housed male mice used for scientific purposes. ABSTRACT: Rearing laboratory mice in groups is important since social isolation after weaning induces brain alterations, which entails behavioral abnormalities in adulthood. Age is an important factor when grouping males of different litters due to inter-male aggressiveness. The aim of this study was to determine whether newly weaned mice could safely be grouped with late juvenile or early and late pubescent mice, and whether cage cleaning, the number of the hosting group members and testosterone plasma levels have any influence. Newly weaned C57BL/6J, CD1, and SCID Beige male mice were systematically grouped with same strain late juvenile, early or late pubescent male mice in clean or dirty cages of 1, 2 or 3 hosting members. We also analyzed plasma testosterone levels at different postnatal days. Our result showed that only strain and hosting male’s age influence agonistic behavior toward newly weaned mice. Thus, in order not to house a recently weaned male alone, we would recommend grouping it with late juvenile same strain mice in all studied strains. In the same way, CD1 and SCID Beige pubescent mice will admit a newly weaned mouse in their group. However, we would not recommend grouping newly weaned and pubescent C57BL/6J males.