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Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1

The Twort-like myoviruses (Kayvirus genus) of S. aureus are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expressi...

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Autores principales: Kornienko, Maria, Fisunov, Gleb, Bespiatykh, Dmitry, Kuptsov, Nikita, Gorodnichev, Roman, Klimina, Ksenia, Kulikov, Eugene, Ilina, Elena, Letarov, Andrey, Shitikov, Egor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698491/
https://www.ncbi.nlm.nih.gov/pubmed/33213043
http://dx.doi.org/10.3390/v12111320
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author Kornienko, Maria
Fisunov, Gleb
Bespiatykh, Dmitry
Kuptsov, Nikita
Gorodnichev, Roman
Klimina, Ksenia
Kulikov, Eugene
Ilina, Elena
Letarov, Andrey
Shitikov, Egor
author_facet Kornienko, Maria
Fisunov, Gleb
Bespiatykh, Dmitry
Kuptsov, Nikita
Gorodnichev, Roman
Klimina, Ksenia
Kulikov, Eugene
Ilina, Elena
Letarov, Andrey
Shitikov, Egor
author_sort Kornienko, Maria
collection PubMed
description The Twort-like myoviruses (Kayvirus genus) of S. aureus are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the Kayvirus genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp).
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spelling pubmed-76984912020-11-29 Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1 Kornienko, Maria Fisunov, Gleb Bespiatykh, Dmitry Kuptsov, Nikita Gorodnichev, Roman Klimina, Ksenia Kulikov, Eugene Ilina, Elena Letarov, Andrey Shitikov, Egor Viruses Article The Twort-like myoviruses (Kayvirus genus) of S. aureus are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the Kayvirus genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp). MDPI 2020-11-17 /pmc/articles/PMC7698491/ /pubmed/33213043 http://dx.doi.org/10.3390/v12111320 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kornienko, Maria
Fisunov, Gleb
Bespiatykh, Dmitry
Kuptsov, Nikita
Gorodnichev, Roman
Klimina, Ksenia
Kulikov, Eugene
Ilina, Elena
Letarov, Andrey
Shitikov, Egor
Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1
title Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1
title_full Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1
title_fullStr Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1
title_full_unstemmed Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1
title_short Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1
title_sort transcriptional landscape of staphylococcus aureus kayvirus bacteriophage vb_saum-515a1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698491/
https://www.ncbi.nlm.nih.gov/pubmed/33213043
http://dx.doi.org/10.3390/v12111320
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