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Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation

Optical mapping is recognized as a promising tool for the registration of electrical activity in the heart. Most cardiac optical mapping experiments are performed in ex vivo isolated heart models. However, the electrophysiological properties of the heart are highly influenced by the autonomic nervou...

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Autores principales: Martišienė, Irma, Mačianskienė, Regina, Benetis, Rimantas, Jurevičius, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698624/
https://www.ncbi.nlm.nih.gov/pubmed/33217906
http://dx.doi.org/10.3390/medicina56110620
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author Martišienė, Irma
Mačianskienė, Regina
Benetis, Rimantas
Jurevičius, Jonas
author_facet Martišienė, Irma
Mačianskienė, Regina
Benetis, Rimantas
Jurevičius, Jonas
author_sort Martišienė, Irma
collection PubMed
description Optical mapping is recognized as a promising tool for the registration of electrical activity in the heart. Most cardiac optical mapping experiments are performed in ex vivo isolated heart models. However, the electrophysiological properties of the heart are highly influenced by the autonomic nervous system as well as humoral regulation; therefore, in vivo investigations of heart activity in large animals are definitely preferred. Furthermore, such investigations can be considered the last step before clinical application. Recently, two comprehensive studies have examined optical mapping approaches for pig hearts in situ (in vivo), likely advancing the methodological capacity to perform complex electrophysiological investigations of the heart. Both studies had the same aim, i.e., to develop high-spatiotemporal-resolution optical mapping suitable for registration of electrical activity of pig heart in situ, but the methods chosen were different. In this brief review, we analyse and compare the results of recent studies and discuss their translational potential for in situ cardiac optical mapping applications in large animals. We focus on the modes of blood circulation that are employed, the use of different voltage-sensitive dyes and their loading procedures, and ways of eliminating contraction artefacts. Finally, we evaluate the possible scenarios for optical mapping (OM) application in large animals in situ and infer which scenario is optimal.
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spelling pubmed-76986242020-11-29 Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation Martišienė, Irma Mačianskienė, Regina Benetis, Rimantas Jurevičius, Jonas Medicina (Kaunas) Review Optical mapping is recognized as a promising tool for the registration of electrical activity in the heart. Most cardiac optical mapping experiments are performed in ex vivo isolated heart models. However, the electrophysiological properties of the heart are highly influenced by the autonomic nervous system as well as humoral regulation; therefore, in vivo investigations of heart activity in large animals are definitely preferred. Furthermore, such investigations can be considered the last step before clinical application. Recently, two comprehensive studies have examined optical mapping approaches for pig hearts in situ (in vivo), likely advancing the methodological capacity to perform complex electrophysiological investigations of the heart. Both studies had the same aim, i.e., to develop high-spatiotemporal-resolution optical mapping suitable for registration of electrical activity of pig heart in situ, but the methods chosen were different. In this brief review, we analyse and compare the results of recent studies and discuss their translational potential for in situ cardiac optical mapping applications in large animals. We focus on the modes of blood circulation that are employed, the use of different voltage-sensitive dyes and their loading procedures, and ways of eliminating contraction artefacts. Finally, we evaluate the possible scenarios for optical mapping (OM) application in large animals in situ and infer which scenario is optimal. MDPI 2020-11-18 /pmc/articles/PMC7698624/ /pubmed/33217906 http://dx.doi.org/10.3390/medicina56110620 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Martišienė, Irma
Mačianskienė, Regina
Benetis, Rimantas
Jurevičius, Jonas
Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation
title Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation
title_full Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation
title_fullStr Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation
title_full_unstemmed Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation
title_short Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation
title_sort cardiac optical mapping in situ in swine models: a view of the current situation
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698624/
https://www.ncbi.nlm.nih.gov/pubmed/33217906
http://dx.doi.org/10.3390/medicina56110620
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