Cargando…

Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan

Background: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging threat in healthcare settings worldwide. Objectives: We evaluated the presence of carbapenemase genes in CPE in a tertiary care university hospital in Tokyo, Japan. Methods: Carbapenem-resistant clinical isolates were colle...

Descripción completa

Detalles Bibliográficos
Autores principales: Nishida, Satoshi, Matsunaga, Naohisa, Kamimura, Yuta, Ishigaki, Shinobu, Furukawa, Taiji, Ono, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698710/
https://www.ncbi.nlm.nih.gov/pubmed/33217991
http://dx.doi.org/10.3390/microorganisms8111816
_version_ 1783615891808714752
author Nishida, Satoshi
Matsunaga, Naohisa
Kamimura, Yuta
Ishigaki, Shinobu
Furukawa, Taiji
Ono, Yasuo
author_facet Nishida, Satoshi
Matsunaga, Naohisa
Kamimura, Yuta
Ishigaki, Shinobu
Furukawa, Taiji
Ono, Yasuo
author_sort Nishida, Satoshi
collection PubMed
description Background: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging threat in healthcare settings worldwide. Objectives: We evaluated the presence of carbapenemase genes in CPE in a tertiary care university hospital in Tokyo, Japan. Methods: Carbapenem-resistant clinical isolates were collected in 2018 at Teikyo University Hospital (Tokyo, Japan). Bacterial species were identified using MALDI-TOF MS. Carbapenemase production was evaluated using a carbapenemase inactivation method. The presence of carbapenemase genes was confirmed by multiplex PCR and DNA sequencing. Results: Four CPE isolates were identified: two Enterobacter cloacae complex strains and Klebsiella oxytoca and Klebsiella pneumoniae strains. Three of the isolates (E. cloacae complex and K. oxytoca) were IMP-1-type producers, including IMP-10 in their produced metallo-β-lactamase, and are epidemic in East Japan. The IMP-10-producing E. cloacae complex strain also produced CTX-M ESBL. The other CPE isolate (K. pneumoniae) is a VIM-1 producer. VIM-1-producing K. pneumoniae is epidemic in Europe, especially in Greece. Accordingly, the VIM-1 producer was isolated from a patient with a medical history in Greece. Conclusions: This study revealed the emergence of E. cloacae complex co-producing IMP-1-type carbapenemase and CTX-M ESBL, and K. pneumoniae producing VIM-1 carbapenemase in clinical isolates in Japan. Metallo-β-lactamase was the most prevalent type of carbapenemase at Teikyo University Hospital, especially IMP-1-type carbapenemase. The detection of VIM-1-producing K. pneumoniae suggests that epidemic CPE from overseas can spread to countries with low CPE prevalence, such as Japan, highlighting the need for active surveillance.
format Online
Article
Text
id pubmed-7698710
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76987102020-11-29 Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan Nishida, Satoshi Matsunaga, Naohisa Kamimura, Yuta Ishigaki, Shinobu Furukawa, Taiji Ono, Yasuo Microorganisms Communication Background: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging threat in healthcare settings worldwide. Objectives: We evaluated the presence of carbapenemase genes in CPE in a tertiary care university hospital in Tokyo, Japan. Methods: Carbapenem-resistant clinical isolates were collected in 2018 at Teikyo University Hospital (Tokyo, Japan). Bacterial species were identified using MALDI-TOF MS. Carbapenemase production was evaluated using a carbapenemase inactivation method. The presence of carbapenemase genes was confirmed by multiplex PCR and DNA sequencing. Results: Four CPE isolates were identified: two Enterobacter cloacae complex strains and Klebsiella oxytoca and Klebsiella pneumoniae strains. Three of the isolates (E. cloacae complex and K. oxytoca) were IMP-1-type producers, including IMP-10 in their produced metallo-β-lactamase, and are epidemic in East Japan. The IMP-10-producing E. cloacae complex strain also produced CTX-M ESBL. The other CPE isolate (K. pneumoniae) is a VIM-1 producer. VIM-1-producing K. pneumoniae is epidemic in Europe, especially in Greece. Accordingly, the VIM-1 producer was isolated from a patient with a medical history in Greece. Conclusions: This study revealed the emergence of E. cloacae complex co-producing IMP-1-type carbapenemase and CTX-M ESBL, and K. pneumoniae producing VIM-1 carbapenemase in clinical isolates in Japan. Metallo-β-lactamase was the most prevalent type of carbapenemase at Teikyo University Hospital, especially IMP-1-type carbapenemase. The detection of VIM-1-producing K. pneumoniae suggests that epidemic CPE from overseas can spread to countries with low CPE prevalence, such as Japan, highlighting the need for active surveillance. MDPI 2020-11-18 /pmc/articles/PMC7698710/ /pubmed/33217991 http://dx.doi.org/10.3390/microorganisms8111816 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nishida, Satoshi
Matsunaga, Naohisa
Kamimura, Yuta
Ishigaki, Shinobu
Furukawa, Taiji
Ono, Yasuo
Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan
title Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan
title_full Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan
title_fullStr Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan
title_full_unstemmed Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan
title_short Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan
title_sort emergence of enterobacter cloacae complex co-producing imp-10 and ctx-m, and klebsiella pneumoniae producing vim-1 in clinical isolates in japan
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698710/
https://www.ncbi.nlm.nih.gov/pubmed/33217991
http://dx.doi.org/10.3390/microorganisms8111816
work_keys_str_mv AT nishidasatoshi emergenceofenterobactercloacaecomplexcoproducingimp10andctxmandklebsiellapneumoniaeproducingvim1inclinicalisolatesinjapan
AT matsunaganaohisa emergenceofenterobactercloacaecomplexcoproducingimp10andctxmandklebsiellapneumoniaeproducingvim1inclinicalisolatesinjapan
AT kamimurayuta emergenceofenterobactercloacaecomplexcoproducingimp10andctxmandklebsiellapneumoniaeproducingvim1inclinicalisolatesinjapan
AT ishigakishinobu emergenceofenterobactercloacaecomplexcoproducingimp10andctxmandklebsiellapneumoniaeproducingvim1inclinicalisolatesinjapan
AT furukawataiji emergenceofenterobactercloacaecomplexcoproducingimp10andctxmandklebsiellapneumoniaeproducingvim1inclinicalisolatesinjapan
AT onoyasuo emergenceofenterobactercloacaecomplexcoproducingimp10andctxmandklebsiellapneumoniaeproducingvim1inclinicalisolatesinjapan