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Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo

Melanogenesis has many important physiological functions. However, abnormal melanin production causes various pigmentation disorders. Melanin synthesis is stimulated by α-melanocyte stimulating hormone (α-MSH) and ultraviolet (UV) irradiation. Lotus seedpod extract (LSE) has been reported as possess...

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Autores principales: Hsu, Jen-Ying, Lin, Hui-Hsuan, Li, Ting-Shuan, Tseng, Chaio-Yun, Wong, Yueching, Chen, Jing-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698734/
https://www.ncbi.nlm.nih.gov/pubmed/33218008
http://dx.doi.org/10.3390/nu12113535
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author Hsu, Jen-Ying
Lin, Hui-Hsuan
Li, Ting-Shuan
Tseng, Chaio-Yun
Wong, Yueching
Chen, Jing-Hsien
author_facet Hsu, Jen-Ying
Lin, Hui-Hsuan
Li, Ting-Shuan
Tseng, Chaio-Yun
Wong, Yueching
Chen, Jing-Hsien
author_sort Hsu, Jen-Ying
collection PubMed
description Melanogenesis has many important physiological functions. However, abnormal melanin production causes various pigmentation disorders. Melanin synthesis is stimulated by α-melanocyte stimulating hormone (α-MSH) and ultraviolet (UV) irradiation. Lotus seedpod extract (LSE) has been reported as possessing antioxidative, anti-aging, and anticancer activities. The present study examined the effect of LSE on melanogenesis and the involved signaling pathways in vitro and in vivo. Results showed that non-cytotoxic doses of LSE and its main component epigallocatechin (EGC) reduced both tyrosinase activity and melanin production in the α-MSH-induced melanoma cells. Western blotting data revealed that LSE and EGC inhibited expressions of tyrosinase and tyrosinase-related protein 1 (TRP-1). Phosphorylation of p38 and protein kinase A (PKA) stimulated by α-MSH was efficiently blocked by LSE treatment. Furthermore, LSE suppressed the nuclear level of cAMP-response element binding protein (CREB) and disturbed the activation of melanocyte inducing transcription factor (MITF) in the α-MSH-stimulated B16F0 cells. The in vivo study revealed that LSE inhibited melanin production in the ear skin of C57BL/6 mice after exposure to UVB. These findings suggested that the anti-melanogenesis of LSE involved both PKA and p38 signaling pathways. LSE is a potent novo natural depigmenting agent for cosmetics or pharmaceutical applications.
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spelling pubmed-76987342020-11-29 Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo Hsu, Jen-Ying Lin, Hui-Hsuan Li, Ting-Shuan Tseng, Chaio-Yun Wong, Yueching Chen, Jing-Hsien Nutrients Article Melanogenesis has many important physiological functions. However, abnormal melanin production causes various pigmentation disorders. Melanin synthesis is stimulated by α-melanocyte stimulating hormone (α-MSH) and ultraviolet (UV) irradiation. Lotus seedpod extract (LSE) has been reported as possessing antioxidative, anti-aging, and anticancer activities. The present study examined the effect of LSE on melanogenesis and the involved signaling pathways in vitro and in vivo. Results showed that non-cytotoxic doses of LSE and its main component epigallocatechin (EGC) reduced both tyrosinase activity and melanin production in the α-MSH-induced melanoma cells. Western blotting data revealed that LSE and EGC inhibited expressions of tyrosinase and tyrosinase-related protein 1 (TRP-1). Phosphorylation of p38 and protein kinase A (PKA) stimulated by α-MSH was efficiently blocked by LSE treatment. Furthermore, LSE suppressed the nuclear level of cAMP-response element binding protein (CREB) and disturbed the activation of melanocyte inducing transcription factor (MITF) in the α-MSH-stimulated B16F0 cells. The in vivo study revealed that LSE inhibited melanin production in the ear skin of C57BL/6 mice after exposure to UVB. These findings suggested that the anti-melanogenesis of LSE involved both PKA and p38 signaling pathways. LSE is a potent novo natural depigmenting agent for cosmetics or pharmaceutical applications. MDPI 2020-11-18 /pmc/articles/PMC7698734/ /pubmed/33218008 http://dx.doi.org/10.3390/nu12113535 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hsu, Jen-Ying
Lin, Hui-Hsuan
Li, Ting-Shuan
Tseng, Chaio-Yun
Wong, Yueching
Chen, Jing-Hsien
Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo
title Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo
title_full Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo
title_fullStr Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo
title_full_unstemmed Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo
title_short Anti-Melanogenesis Effects of Lotus Seedpod In Vitro and In Vivo
title_sort anti-melanogenesis effects of lotus seedpod in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698734/
https://www.ncbi.nlm.nih.gov/pubmed/33218008
http://dx.doi.org/10.3390/nu12113535
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