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Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis

Background: The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have signif...

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Autores principales: Imoto, Yoshimasa, Takabayashi, Tetsuji, Sakashita, Masafumi, Kato, Yukinori, Yoshida, Kanako, Kidoguchi, Masanori, Koyama, Keisuke, Adachi, Naoto, Kimura, Yukihiro, Ogi, Kazuhiro, Ito, Yumi, Kanno, Masafumi, Okamoto, Masayuki, Narita, Norihiko, Fujieda, Shigeharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698943/
https://www.ncbi.nlm.nih.gov/pubmed/33218117
http://dx.doi.org/10.3390/biom10111568
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author Imoto, Yoshimasa
Takabayashi, Tetsuji
Sakashita, Masafumi
Kato, Yukinori
Yoshida, Kanako
Kidoguchi, Masanori
Koyama, Keisuke
Adachi, Naoto
Kimura, Yukihiro
Ogi, Kazuhiro
Ito, Yumi
Kanno, Masafumi
Okamoto, Masayuki
Narita, Norihiko
Fujieda, Shigeharu
author_facet Imoto, Yoshimasa
Takabayashi, Tetsuji
Sakashita, Masafumi
Kato, Yukinori
Yoshida, Kanako
Kidoguchi, Masanori
Koyama, Keisuke
Adachi, Naoto
Kimura, Yukihiro
Ogi, Kazuhiro
Ito, Yumi
Kanno, Masafumi
Okamoto, Masayuki
Narita, Norihiko
Fujieda, Shigeharu
author_sort Imoto, Yoshimasa
collection PubMed
description Background: The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have significant roles in allergic disease; however, the significance of 15-LOX-1 in CRS is not well understood. The objective of this study was to demonstrate the expression of 15-LOX-1 in CRS. Methods: The mRNA expression levels of 15-LOX-1 and periostin in nasal tissues were measured by quantitative real-time polymerase chain reaction. We also performed an immunofluorescence study of nasal tissues. Cells of the Eol-1 eosinophilic leukemic cell line were stimulated with interleukin-33 to test the induction of 15-LOX-1. Results: The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Moreover, interleukin-33 enhanced 15-LOX-1 expression in Eol-1 cells. Conclusions: 15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS.
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spelling pubmed-76989432020-11-29 Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis Imoto, Yoshimasa Takabayashi, Tetsuji Sakashita, Masafumi Kato, Yukinori Yoshida, Kanako Kidoguchi, Masanori Koyama, Keisuke Adachi, Naoto Kimura, Yukihiro Ogi, Kazuhiro Ito, Yumi Kanno, Masafumi Okamoto, Masayuki Narita, Norihiko Fujieda, Shigeharu Biomolecules Article Background: The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have significant roles in allergic disease; however, the significance of 15-LOX-1 in CRS is not well understood. The objective of this study was to demonstrate the expression of 15-LOX-1 in CRS. Methods: The mRNA expression levels of 15-LOX-1 and periostin in nasal tissues were measured by quantitative real-time polymerase chain reaction. We also performed an immunofluorescence study of nasal tissues. Cells of the Eol-1 eosinophilic leukemic cell line were stimulated with interleukin-33 to test the induction of 15-LOX-1. Results: The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Moreover, interleukin-33 enhanced 15-LOX-1 expression in Eol-1 cells. Conclusions: 15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS. MDPI 2020-11-18 /pmc/articles/PMC7698943/ /pubmed/33218117 http://dx.doi.org/10.3390/biom10111568 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Imoto, Yoshimasa
Takabayashi, Tetsuji
Sakashita, Masafumi
Kato, Yukinori
Yoshida, Kanako
Kidoguchi, Masanori
Koyama, Keisuke
Adachi, Naoto
Kimura, Yukihiro
Ogi, Kazuhiro
Ito, Yumi
Kanno, Masafumi
Okamoto, Masayuki
Narita, Norihiko
Fujieda, Shigeharu
Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
title Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
title_full Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
title_fullStr Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
title_full_unstemmed Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
title_short Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
title_sort enhanced 15-lipoxygenase 1 production is related to periostin expression and eosinophil recruitment in eosinophilic chronic rhinosinusitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698943/
https://www.ncbi.nlm.nih.gov/pubmed/33218117
http://dx.doi.org/10.3390/biom10111568
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