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Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows

The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic acid and lauric and caprylic saturated fatty acids. Eleven mastitis pathogens were isolated from sub-clinical and clinical bovine mastitis cases for the study. An initial scre...

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Autores principales: Pangprasit, Noppason, Srithanasuwan, Anyaphat, Suriyasathaporn, Witaya, Pikulkaew, Surachai, Bernard, John K., Chaisri, Wasana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699284/
https://www.ncbi.nlm.nih.gov/pubmed/33228007
http://dx.doi.org/10.3390/pathogens9110961
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author Pangprasit, Noppason
Srithanasuwan, Anyaphat
Suriyasathaporn, Witaya
Pikulkaew, Surachai
Bernard, John K.
Chaisri, Wasana
author_facet Pangprasit, Noppason
Srithanasuwan, Anyaphat
Suriyasathaporn, Witaya
Pikulkaew, Surachai
Bernard, John K.
Chaisri, Wasana
author_sort Pangprasit, Noppason
collection PubMed
description The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic acid and lauric and caprylic saturated fatty acids. Eleven mastitis pathogens were isolated from sub-clinical and clinical bovine mastitis cases for the study. An initial screening of their antibacterial activities by agar well diffusion method was performed. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each acid were obtained using a microdilution method; each acid was diluted from stock solution and then were diluted with culture broth to reach concentrations ranging from 4 to 0.004% w/v. The results showed acetic acid had the highest zone of inhibition against all pathogens except Escherichia coli compared with lauric and caprylic acids. The MIC and MBC were lowest for acetic acid against both Gram-positive (except Staphylococcus chromogenes from the coagulase negative staphylococci (CNS) group) and Gram-negative pathogens, intermediate for lactic and caprylic acids and greatest for lauric acid. In conclusion, acetic acid had antimicrobial activities against most mastitis pathogens compared with other acids. Further studies are needed to optimize the formulation and concentration of acetic acid for teat-dipping agent in the future.
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spelling pubmed-76992842020-11-29 Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows Pangprasit, Noppason Srithanasuwan, Anyaphat Suriyasathaporn, Witaya Pikulkaew, Surachai Bernard, John K. Chaisri, Wasana Pathogens Communication The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic acid and lauric and caprylic saturated fatty acids. Eleven mastitis pathogens were isolated from sub-clinical and clinical bovine mastitis cases for the study. An initial screening of their antibacterial activities by agar well diffusion method was performed. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each acid were obtained using a microdilution method; each acid was diluted from stock solution and then were diluted with culture broth to reach concentrations ranging from 4 to 0.004% w/v. The results showed acetic acid had the highest zone of inhibition against all pathogens except Escherichia coli compared with lauric and caprylic acids. The MIC and MBC were lowest for acetic acid against both Gram-positive (except Staphylococcus chromogenes from the coagulase negative staphylococci (CNS) group) and Gram-negative pathogens, intermediate for lactic and caprylic acids and greatest for lauric acid. In conclusion, acetic acid had antimicrobial activities against most mastitis pathogens compared with other acids. Further studies are needed to optimize the formulation and concentration of acetic acid for teat-dipping agent in the future. MDPI 2020-11-19 /pmc/articles/PMC7699284/ /pubmed/33228007 http://dx.doi.org/10.3390/pathogens9110961 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Pangprasit, Noppason
Srithanasuwan, Anyaphat
Suriyasathaporn, Witaya
Pikulkaew, Surachai
Bernard, John K.
Chaisri, Wasana
Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
title Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
title_full Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
title_fullStr Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
title_full_unstemmed Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
title_short Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
title_sort antibacterial activities of acetic acid against major and minor pathogens isolated from mastitis in dairy cows
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699284/
https://www.ncbi.nlm.nih.gov/pubmed/33228007
http://dx.doi.org/10.3390/pathogens9110961
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