A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain

The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for e...

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Autores principales: Cibecchini, Giulia, Cecere, Paola, Tumino, Giorgio, Morcia, Caterina, Ghizzoni, Roberta, Carnevali, Paola, Terzi, Valeria, Pompa, Pier Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699333/
https://www.ncbi.nlm.nih.gov/pubmed/33228015
http://dx.doi.org/10.3390/foods9111691
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author Cibecchini, Giulia
Cecere, Paola
Tumino, Giorgio
Morcia, Caterina
Ghizzoni, Roberta
Carnevali, Paola
Terzi, Valeria
Pompa, Pier Paolo
author_facet Cibecchini, Giulia
Cecere, Paola
Tumino, Giorgio
Morcia, Caterina
Ghizzoni, Roberta
Carnevali, Paola
Terzi, Valeria
Pompa, Pier Paolo
author_sort Cibecchini, Giulia
collection PubMed
description The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for easy and clear identification of specific wheat varieties was developed. Notably, an approach based on the loop-mediated isothermal amplification reaction (LAMP) as an SNP discrimination tool, in combination with naked-eye visualization of the results, was designed and optimized. Our assay was proven to be effective in the detection of adulterated food products, including both substitution and mixing with different crop varieties.
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spelling pubmed-76993332020-11-29 A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain Cibecchini, Giulia Cecere, Paola Tumino, Giorgio Morcia, Caterina Ghizzoni, Roberta Carnevali, Paola Terzi, Valeria Pompa, Pier Paolo Foods Article The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for easy and clear identification of specific wheat varieties was developed. Notably, an approach based on the loop-mediated isothermal amplification reaction (LAMP) as an SNP discrimination tool, in combination with naked-eye visualization of the results, was designed and optimized. Our assay was proven to be effective in the detection of adulterated food products, including both substitution and mixing with different crop varieties. MDPI 2020-11-19 /pmc/articles/PMC7699333/ /pubmed/33228015 http://dx.doi.org/10.3390/foods9111691 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cibecchini, Giulia
Cecere, Paola
Tumino, Giorgio
Morcia, Caterina
Ghizzoni, Roberta
Carnevali, Paola
Terzi, Valeria
Pompa, Pier Paolo
A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
title A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
title_full A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
title_fullStr A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
title_full_unstemmed A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
title_short A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
title_sort fast, naked-eye assay for varietal traceability in the durum wheat production chain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699333/
https://www.ncbi.nlm.nih.gov/pubmed/33228015
http://dx.doi.org/10.3390/foods9111691
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