Highly Efficient Antimicrobial Activity of Cu(x)Fe(y)O(z) Nanoparticles against Important Human Pathogens

The development of innovative antimicrobial materials is crucial in thwarting infectious diseases caused by microbes, as drug-resistant pathogens are increasing in both number and capacity to detoxify the antimicrobial drugs used today. An ideal antimicrobial material should inhibit a wide variety of bacteria in a short period of time, be less or not toxic to normal cells, and the fabrication or synthesis process should be cheap and easy. We report a one-step microwave-assisted hydrothermal synthesis of mixed composite Cu(x)Fe(y)O(z) (Fe(2)O(3)/Cu(2)O/CuO/CuFe(2)O) nanoparticles (NPs) as an excellent antimicrobial material. The 1 mg/mL Cu(x)Fe(y)O(z) NPs with the composition 36% CuFeO(2), 28% Cu(2)O and 36% Fe(2)O(3) have a general antimicrobial activity greater than 5 log reduction within 4 h against nine important human pathogenic bacteria (including drug-resistant bacteria as well as Gram-positive and Gram-negative strains). For example, they induced a >9 log reduction in Escherichia coli B viability after 15 min of incubation, and an ~8 log reduction in multidrug-resistant Klebsiella pneumoniae after 4 h incubation. Cytotoxicity tests against mouse fibroblast cells showed about 74% viability when exposed to 1 mg/mL Cu(x)Fe(y)O(z) NPs for 24 h, compared to the 20% viability for 1 mg/mL pure Cu(2)O NPs synthesized by the same method. These results show that the Cu(x)Fe(y)O(z) composite NPs are a highly efficient, low-toxicity and cheap antimicrobial material that has promising potential for applications in medical and food safety.