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Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples

Plant viruses are important pathogens that cause significant crop losses. A plant protein extraction protocol that combines crushing the tissue by a pestle in liquid nitrogen with subsequent crushing by a roller-ball crusher in urea solution, followed by RuBisCO depletion, reduction, alkylation, pro...

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Detalles Bibliográficos
Autores principales:	Cejnar, Pavel, Kučková, Štěpánka, Šantrůček, Jiří, Glasa, Miroslav, Komínek, Petr, Mihálik, Daniel, Slavíková, Lucie, Leišová-Svobodová, Leona, Smirnova, Tatiana, Hynek, Radovan, Kundu, Jiban Kumar, Ryšánek, Pavel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699591/ https://www.ncbi.nlm.nih.gov/pubmed/33228257 http://dx.doi.org/10.3390/pathogens9110966

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