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Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient
The current study was aimed to evaluate the phenolic composition parameters of two hydro-alcoholic extracts of Ocimum basilicum L. (OB) obtained from the aerial part (without leaves) and leaves, in order to determine their contribution to the antioxidant activity (AOA). Both hydro-alcoholic extracts...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699777/ https://www.ncbi.nlm.nih.gov/pubmed/33233640 http://dx.doi.org/10.3390/molecules25225442 |
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author | Faur, Alin Watz, Claudia Moacă, Elena-Alina Avram, Ştefana Borcan, Florin Pinzaru, Iulia Iftode, Andrada Nicolov, Mirela Popovici, Ramona Amina Raica, Marius Szuhanek, Camelia A. Dehelean, Cristina |
author_facet | Faur, Alin Watz, Claudia Moacă, Elena-Alina Avram, Ştefana Borcan, Florin Pinzaru, Iulia Iftode, Andrada Nicolov, Mirela Popovici, Ramona Amina Raica, Marius Szuhanek, Camelia A. Dehelean, Cristina |
author_sort | Faur, Alin |
collection | PubMed |
description | The current study was aimed to evaluate the phenolic composition parameters of two hydro-alcoholic extracts of Ocimum basilicum L. (OB) obtained from the aerial part (without leaves) and leaves, in order to determine their contribution to the antioxidant activity (AOA). Both hydro-alcoholic extracts have proven to be rich in polyphenolic compounds, flavonoids, flavonols and tannins. Therefore, the leaves’ extracts reveal an inhibition percentage of 89%, almost comparable with the standard reference (95%). To complete the toxicological profile, the study also assessed the potential cytotoxicity of basil hydro-alcoholic extracts on immortalized human keratinocytes (HaCaT), skin human fibroblasts (1BR3), mice epidermis (JB6Cl41-5a) and primary human melanocytes (HEMa) cells, correlated to A375 antitumor in vitro activity. The extracts did not induce significant cytotoxic effect on any of the selected normal cell lines but showed relevant activity on A375 cells. Considering the low values obtained regarding the irritative effects in the chorionallantoic membrane of the egg on blood vessels, we can emphasize that both extracts can be considered as biocompatible ingredients. Regarding the potential activity of hydro-alcoholic extracts on human skin, the decrease of erythema values after the application of extracts was a relevant observation which indicates the anti-inflammatory potential of Ocimum basilicum L. |
format | Online Article Text |
id | pubmed-7699777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76997772020-11-29 Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient Faur, Alin Watz, Claudia Moacă, Elena-Alina Avram, Ştefana Borcan, Florin Pinzaru, Iulia Iftode, Andrada Nicolov, Mirela Popovici, Ramona Amina Raica, Marius Szuhanek, Camelia A. Dehelean, Cristina Molecules Article The current study was aimed to evaluate the phenolic composition parameters of two hydro-alcoholic extracts of Ocimum basilicum L. (OB) obtained from the aerial part (without leaves) and leaves, in order to determine their contribution to the antioxidant activity (AOA). Both hydro-alcoholic extracts have proven to be rich in polyphenolic compounds, flavonoids, flavonols and tannins. Therefore, the leaves’ extracts reveal an inhibition percentage of 89%, almost comparable with the standard reference (95%). To complete the toxicological profile, the study also assessed the potential cytotoxicity of basil hydro-alcoholic extracts on immortalized human keratinocytes (HaCaT), skin human fibroblasts (1BR3), mice epidermis (JB6Cl41-5a) and primary human melanocytes (HEMa) cells, correlated to A375 antitumor in vitro activity. The extracts did not induce significant cytotoxic effect on any of the selected normal cell lines but showed relevant activity on A375 cells. Considering the low values obtained regarding the irritative effects in the chorionallantoic membrane of the egg on blood vessels, we can emphasize that both extracts can be considered as biocompatible ingredients. Regarding the potential activity of hydro-alcoholic extracts on human skin, the decrease of erythema values after the application of extracts was a relevant observation which indicates the anti-inflammatory potential of Ocimum basilicum L. MDPI 2020-11-20 /pmc/articles/PMC7699777/ /pubmed/33233640 http://dx.doi.org/10.3390/molecules25225442 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Faur, Alin Watz, Claudia Moacă, Elena-Alina Avram, Ştefana Borcan, Florin Pinzaru, Iulia Iftode, Andrada Nicolov, Mirela Popovici, Ramona Amina Raica, Marius Szuhanek, Camelia A. Dehelean, Cristina Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient |
title | Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient |
title_full | Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient |
title_fullStr | Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient |
title_full_unstemmed | Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient |
title_short | Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient |
title_sort | correlations on phenolic screening related to in vitro and in ovo assessment of ocimum basilicum l. hydro-alcoholic extracts used as skin active ingredient |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699777/ https://www.ncbi.nlm.nih.gov/pubmed/33233640 http://dx.doi.org/10.3390/molecules25225442 |
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