Cargando…
Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs
Sterigmatocystin (STC) and 5-methoxysterigmatocystin (5-M-STC) are mycotoxins produced by common damp indoor Aspergilli series Versicolores. Since both STC and 5-M-STC were found in the dust of indoor occupational and living areas, their occupants may be exposed to these mycotoxins, primarily by inh...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700161/ https://www.ncbi.nlm.nih.gov/pubmed/33238460 http://dx.doi.org/10.3390/toxins12110734 |
_version_ | 1783616214325526528 |
---|---|
author | Jakšić, Daniela Ćurtović, Ida Kifer, Domagoj Rašić, Dubravka Kopjar, Nevenka Micek, Vedran Peraica, Maja Klarić, Maja Šegvić |
author_facet | Jakšić, Daniela Ćurtović, Ida Kifer, Domagoj Rašić, Dubravka Kopjar, Nevenka Micek, Vedran Peraica, Maja Klarić, Maja Šegvić |
author_sort | Jakšić, Daniela |
collection | PubMed |
description | Sterigmatocystin (STC) and 5-methoxysterigmatocystin (5-M-STC) are mycotoxins produced by common damp indoor Aspergilli series Versicolores. Since both STC and 5-M-STC were found in the dust of indoor occupational and living areas, their occupants may be exposed to these mycotoxins, primarily by inhalation. Thus, STC and 5-M-STC were intratracheally instilled in male Wistar rats using doses (0.3 mg STC/kg of lung weight (l.w.); 3.6 mg 5-M-STC/kg l.w.; toxin combination 0.3 + 3.6 mg/kg l.w.) that corresponded to concentrations detected in the dust of damp indoor areas in order to explore cytotoxicity, vascular permeability, immunomodulation and genotoxicity. Single mycotoxins and their combinations insignificantly altered lactate-dehydrogenase activity, albumin, interleukin-6, tumor necrosis factor-α and chemokine macrophage inflammatory protein-1α concentrations, as measured by ELISA in bronchioalveolar lavage fluid upon 24 h of treatment. In an alkaline comet assay, both mycotoxins provoked a similar intensity of DNA damage in rat lungs, while in a neutral comet assay, only 5-M-STC evoked significant DNA damage. Hence, naturally occurring concentrations of individual STC may induce DNA damage in rat lungs, in which single DNA strand breaks prevail, while 5-M-STC was more responsible for double-strand breaks. In both versions of the comet assay treatment with STC + 5-M-STC, less DNA damage intensity occurred compared to single mycotoxin treatment, suggesting an antagonistic genotoxic action. |
format | Online Article Text |
id | pubmed-7700161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77001612020-11-30 Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs Jakšić, Daniela Ćurtović, Ida Kifer, Domagoj Rašić, Dubravka Kopjar, Nevenka Micek, Vedran Peraica, Maja Klarić, Maja Šegvić Toxins (Basel) Article Sterigmatocystin (STC) and 5-methoxysterigmatocystin (5-M-STC) are mycotoxins produced by common damp indoor Aspergilli series Versicolores. Since both STC and 5-M-STC were found in the dust of indoor occupational and living areas, their occupants may be exposed to these mycotoxins, primarily by inhalation. Thus, STC and 5-M-STC were intratracheally instilled in male Wistar rats using doses (0.3 mg STC/kg of lung weight (l.w.); 3.6 mg 5-M-STC/kg l.w.; toxin combination 0.3 + 3.6 mg/kg l.w.) that corresponded to concentrations detected in the dust of damp indoor areas in order to explore cytotoxicity, vascular permeability, immunomodulation and genotoxicity. Single mycotoxins and their combinations insignificantly altered lactate-dehydrogenase activity, albumin, interleukin-6, tumor necrosis factor-α and chemokine macrophage inflammatory protein-1α concentrations, as measured by ELISA in bronchioalveolar lavage fluid upon 24 h of treatment. In an alkaline comet assay, both mycotoxins provoked a similar intensity of DNA damage in rat lungs, while in a neutral comet assay, only 5-M-STC evoked significant DNA damage. Hence, naturally occurring concentrations of individual STC may induce DNA damage in rat lungs, in which single DNA strand breaks prevail, while 5-M-STC was more responsible for double-strand breaks. In both versions of the comet assay treatment with STC + 5-M-STC, less DNA damage intensity occurred compared to single mycotoxin treatment, suggesting an antagonistic genotoxic action. MDPI 2020-11-23 /pmc/articles/PMC7700161/ /pubmed/33238460 http://dx.doi.org/10.3390/toxins12110734 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jakšić, Daniela Ćurtović, Ida Kifer, Domagoj Rašić, Dubravka Kopjar, Nevenka Micek, Vedran Peraica, Maja Klarić, Maja Šegvić Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs |
title | Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs |
title_full | Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs |
title_fullStr | Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs |
title_full_unstemmed | Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs |
title_short | Single-Dose Toxicity of Individual and Combined Sterigmatocystin and 5-Methoxysterigmatocistin in Rat Lungs |
title_sort | single-dose toxicity of individual and combined sterigmatocystin and 5-methoxysterigmatocistin in rat lungs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700161/ https://www.ncbi.nlm.nih.gov/pubmed/33238460 http://dx.doi.org/10.3390/toxins12110734 |
work_keys_str_mv | AT jaksicdaniela singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT curtovicida singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT kiferdomagoj singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT rasicdubravka singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT kopjarnevenka singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT micekvedran singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT peraicamaja singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs AT klaricmajasegvic singledosetoxicityofindividualandcombinedsterigmatocystinand5methoxysterigmatocistininratlungs |