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Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700190/ https://www.ncbi.nlm.nih.gov/pubmed/33238447 http://dx.doi.org/10.3390/plants9111624 |
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author | Cappai, Francesco Garcia, Alexandria Cullen, Ryan Davis, Matthew Munoz, Patricio R. |
author_facet | Cappai, Francesco Garcia, Alexandria Cullen, Ryan Davis, Matthew Munoz, Patricio R. |
author_sort | Cappai, Francesco |
collection | PubMed |
description | The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants. |
format | Online Article Text |
id | pubmed-7700190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77001902020-11-30 Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices Cappai, Francesco Garcia, Alexandria Cullen, Ryan Davis, Matthew Munoz, Patricio R. Plants (Basel) Article The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants. MDPI 2020-11-23 /pmc/articles/PMC7700190/ /pubmed/33238447 http://dx.doi.org/10.3390/plants9111624 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cappai, Francesco Garcia, Alexandria Cullen, Ryan Davis, Matthew Munoz, Patricio R. Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices |
title | Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices |
title_full | Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices |
title_fullStr | Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices |
title_full_unstemmed | Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices |
title_short | Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices |
title_sort | advancements in low-chill blueberry vaccinium corymbosum l. tissue culture practices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700190/ https://www.ncbi.nlm.nih.gov/pubmed/33238447 http://dx.doi.org/10.3390/plants9111624 |
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