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Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices

The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, t...

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Detalles Bibliográficos
Autores principales: Cappai, Francesco, Garcia, Alexandria, Cullen, Ryan, Davis, Matthew, Munoz, Patricio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700190/
https://www.ncbi.nlm.nih.gov/pubmed/33238447
http://dx.doi.org/10.3390/plants9111624
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author Cappai, Francesco
Garcia, Alexandria
Cullen, Ryan
Davis, Matthew
Munoz, Patricio R.
author_facet Cappai, Francesco
Garcia, Alexandria
Cullen, Ryan
Davis, Matthew
Munoz, Patricio R.
author_sort Cappai, Francesco
collection PubMed
description The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants.
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spelling pubmed-77001902020-11-30 Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices Cappai, Francesco Garcia, Alexandria Cullen, Ryan Davis, Matthew Munoz, Patricio R. Plants (Basel) Article The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants. MDPI 2020-11-23 /pmc/articles/PMC7700190/ /pubmed/33238447 http://dx.doi.org/10.3390/plants9111624 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cappai, Francesco
Garcia, Alexandria
Cullen, Ryan
Davis, Matthew
Munoz, Patricio R.
Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
title Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
title_full Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
title_fullStr Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
title_full_unstemmed Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
title_short Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
title_sort advancements in low-chill blueberry vaccinium corymbosum l. tissue culture practices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700190/
https://www.ncbi.nlm.nih.gov/pubmed/33238447
http://dx.doi.org/10.3390/plants9111624
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