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Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
In recent times, many approaches have been developed against drug resistant Gram-negative bacteria. However, low-cost high effective materials which could broaden the spectrum of antibiotics are still needed. In this study, enhancement of linezolid spectrum, normally active against Gram-positive bac...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700326/ https://www.ncbi.nlm.nih.gov/pubmed/33233423 http://dx.doi.org/10.3390/pharmaceutics12111126 |
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author | Guzel Kaya, Gulcihan Medaglia, Serena Candela-Noguera, Vicente Tormo-Mas, María Ángeles Marcos, María Dolores Aznar, Elena Deveci, Huseyin Martínez-Máñez, Ramón |
author_facet | Guzel Kaya, Gulcihan Medaglia, Serena Candela-Noguera, Vicente Tormo-Mas, María Ángeles Marcos, María Dolores Aznar, Elena Deveci, Huseyin Martínez-Máñez, Ramón |
author_sort | Guzel Kaya, Gulcihan |
collection | PubMed |
description | In recent times, many approaches have been developed against drug resistant Gram-negative bacteria. However, low-cost high effective materials which could broaden the spectrum of antibiotics are still needed. In this study, enhancement of linezolid spectrum, normally active against Gram-positive bacteria, was aimed for Gram-negative bacteria growth inhibition. For this purpose, a silica xerogel prepared from a low-cost precursor is used as a drug carrier owing to the advantages of its mesoporous structure, suitable pore and particle size and ultralow density. The silica xerogel is loaded with linezolid and capped with ε-poly-l-lysine. The developed nano-formulation shows a marked antibacterial activity against to Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In comparison to free linezolid and ε-poly-l-lysine, the material demonstrates a synergistic effect on killing for the three tested bacteria. The results show that silica xerogels can be used as a potential drug carrier and activity enhancer. This strategy could provide the improvement of antibacterial activity spectrum of antibacterial agents like linezolid and could represent a powerful alternative to overcome antibiotic resistance in a near future. |
format | Online Article Text |
id | pubmed-7700326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77003262020-11-30 Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier Guzel Kaya, Gulcihan Medaglia, Serena Candela-Noguera, Vicente Tormo-Mas, María Ángeles Marcos, María Dolores Aznar, Elena Deveci, Huseyin Martínez-Máñez, Ramón Pharmaceutics Article In recent times, many approaches have been developed against drug resistant Gram-negative bacteria. However, low-cost high effective materials which could broaden the spectrum of antibiotics are still needed. In this study, enhancement of linezolid spectrum, normally active against Gram-positive bacteria, was aimed for Gram-negative bacteria growth inhibition. For this purpose, a silica xerogel prepared from a low-cost precursor is used as a drug carrier owing to the advantages of its mesoporous structure, suitable pore and particle size and ultralow density. The silica xerogel is loaded with linezolid and capped with ε-poly-l-lysine. The developed nano-formulation shows a marked antibacterial activity against to Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In comparison to free linezolid and ε-poly-l-lysine, the material demonstrates a synergistic effect on killing for the three tested bacteria. The results show that silica xerogels can be used as a potential drug carrier and activity enhancer. This strategy could provide the improvement of antibacterial activity spectrum of antibacterial agents like linezolid and could represent a powerful alternative to overcome antibiotic resistance in a near future. MDPI 2020-11-21 /pmc/articles/PMC7700326/ /pubmed/33233423 http://dx.doi.org/10.3390/pharmaceutics12111126 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guzel Kaya, Gulcihan Medaglia, Serena Candela-Noguera, Vicente Tormo-Mas, María Ángeles Marcos, María Dolores Aznar, Elena Deveci, Huseyin Martínez-Máñez, Ramón Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier |
title | Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier |
title_full | Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier |
title_fullStr | Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier |
title_full_unstemmed | Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier |
title_short | Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier |
title_sort | antibacterial activity of linezolid against gram-negative bacteria: utilization of ε-poly-l-lysine capped silica xerogel as an activating carrier |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700326/ https://www.ncbi.nlm.nih.gov/pubmed/33233423 http://dx.doi.org/10.3390/pharmaceutics12111126 |
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