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Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier

In recent times, many approaches have been developed against drug resistant Gram-negative bacteria. However, low-cost high effective materials which could broaden the spectrum of antibiotics are still needed. In this study, enhancement of linezolid spectrum, normally active against Gram-positive bac...

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Autores principales: Guzel Kaya, Gulcihan, Medaglia, Serena, Candela-Noguera, Vicente, Tormo-Mas, María Ángeles, Marcos, María Dolores, Aznar, Elena, Deveci, Huseyin, Martínez-Máñez, Ramón
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700326/
https://www.ncbi.nlm.nih.gov/pubmed/33233423
http://dx.doi.org/10.3390/pharmaceutics12111126
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author Guzel Kaya, Gulcihan
Medaglia, Serena
Candela-Noguera, Vicente
Tormo-Mas, María Ángeles
Marcos, María Dolores
Aznar, Elena
Deveci, Huseyin
Martínez-Máñez, Ramón
author_facet Guzel Kaya, Gulcihan
Medaglia, Serena
Candela-Noguera, Vicente
Tormo-Mas, María Ángeles
Marcos, María Dolores
Aznar, Elena
Deveci, Huseyin
Martínez-Máñez, Ramón
author_sort Guzel Kaya, Gulcihan
collection PubMed
description In recent times, many approaches have been developed against drug resistant Gram-negative bacteria. However, low-cost high effective materials which could broaden the spectrum of antibiotics are still needed. In this study, enhancement of linezolid spectrum, normally active against Gram-positive bacteria, was aimed for Gram-negative bacteria growth inhibition. For this purpose, a silica xerogel prepared from a low-cost precursor is used as a drug carrier owing to the advantages of its mesoporous structure, suitable pore and particle size and ultralow density. The silica xerogel is loaded with linezolid and capped with ε-poly-l-lysine. The developed nano-formulation shows a marked antibacterial activity against to Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In comparison to free linezolid and ε-poly-l-lysine, the material demonstrates a synergistic effect on killing for the three tested bacteria. The results show that silica xerogels can be used as a potential drug carrier and activity enhancer. This strategy could provide the improvement of antibacterial activity spectrum of antibacterial agents like linezolid and could represent a powerful alternative to overcome antibiotic resistance in a near future.
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spelling pubmed-77003262020-11-30 Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier Guzel Kaya, Gulcihan Medaglia, Serena Candela-Noguera, Vicente Tormo-Mas, María Ángeles Marcos, María Dolores Aznar, Elena Deveci, Huseyin Martínez-Máñez, Ramón Pharmaceutics Article In recent times, many approaches have been developed against drug resistant Gram-negative bacteria. However, low-cost high effective materials which could broaden the spectrum of antibiotics are still needed. In this study, enhancement of linezolid spectrum, normally active against Gram-positive bacteria, was aimed for Gram-negative bacteria growth inhibition. For this purpose, a silica xerogel prepared from a low-cost precursor is used as a drug carrier owing to the advantages of its mesoporous structure, suitable pore and particle size and ultralow density. The silica xerogel is loaded with linezolid and capped with ε-poly-l-lysine. The developed nano-formulation shows a marked antibacterial activity against to Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In comparison to free linezolid and ε-poly-l-lysine, the material demonstrates a synergistic effect on killing for the three tested bacteria. The results show that silica xerogels can be used as a potential drug carrier and activity enhancer. This strategy could provide the improvement of antibacterial activity spectrum of antibacterial agents like linezolid and could represent a powerful alternative to overcome antibiotic resistance in a near future. MDPI 2020-11-21 /pmc/articles/PMC7700326/ /pubmed/33233423 http://dx.doi.org/10.3390/pharmaceutics12111126 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guzel Kaya, Gulcihan
Medaglia, Serena
Candela-Noguera, Vicente
Tormo-Mas, María Ángeles
Marcos, María Dolores
Aznar, Elena
Deveci, Huseyin
Martínez-Máñez, Ramón
Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
title Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
title_full Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
title_fullStr Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
title_full_unstemmed Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
title_short Antibacterial Activity of Linezolid against Gram-Negative Bacteria: Utilization of ε-Poly-l-Lysine Capped Silica Xerogel as an Activating Carrier
title_sort antibacterial activity of linezolid against gram-negative bacteria: utilization of ε-poly-l-lysine capped silica xerogel as an activating carrier
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700326/
https://www.ncbi.nlm.nih.gov/pubmed/33233423
http://dx.doi.org/10.3390/pharmaceutics12111126
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