Cargando…

Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis

Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were c...

Descripción completa

Detalles Bibliográficos
Autores principales: Scutari, Rossana, Piermatteo, Lorenzo, Manuelli, Matteo Ciancio, Iannetta, Marco, Salpini, Romina, Bertoli, Ada, Alteri, Claudia, Saccomandi, Patrizia, Bellocchi, Maria Concetta, Malagnino, Vincenzo, Teti, Elisabetta, Sforza, Daniele, Siragusa, Leandro, Grande, Michele, Sarmati, Loredana, Svicher, Valentina, Andreoni, Massimo, Ceccherini-Silberstein, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700357/
https://www.ncbi.nlm.nih.gov/pubmed/33238410
http://dx.doi.org/10.3390/life10110302
_version_ 1783616260710334464
author Scutari, Rossana
Piermatteo, Lorenzo
Manuelli, Matteo Ciancio
Iannetta, Marco
Salpini, Romina
Bertoli, Ada
Alteri, Claudia
Saccomandi, Patrizia
Bellocchi, Maria Concetta
Malagnino, Vincenzo
Teti, Elisabetta
Sforza, Daniele
Siragusa, Leandro
Grande, Michele
Sarmati, Loredana
Svicher, Valentina
Andreoni, Massimo
Ceccherini-Silberstein, Francesca
author_facet Scutari, Rossana
Piermatteo, Lorenzo
Manuelli, Matteo Ciancio
Iannetta, Marco
Salpini, Romina
Bertoli, Ada
Alteri, Claudia
Saccomandi, Patrizia
Bellocchi, Maria Concetta
Malagnino, Vincenzo
Teti, Elisabetta
Sforza, Daniele
Siragusa, Leandro
Grande, Michele
Sarmati, Loredana
Svicher, Valentina
Andreoni, Massimo
Ceccherini-Silberstein, Francesca
author_sort Scutari, Rossana
collection PubMed
description Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations.
format Online
Article
Text
id pubmed-7700357
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77003572020-11-30 Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis Scutari, Rossana Piermatteo, Lorenzo Manuelli, Matteo Ciancio Iannetta, Marco Salpini, Romina Bertoli, Ada Alteri, Claudia Saccomandi, Patrizia Bellocchi, Maria Concetta Malagnino, Vincenzo Teti, Elisabetta Sforza, Daniele Siragusa, Leandro Grande, Michele Sarmati, Loredana Svicher, Valentina Andreoni, Massimo Ceccherini-Silberstein, Francesca Life (Basel) Case Report Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations. MDPI 2020-11-23 /pmc/articles/PMC7700357/ /pubmed/33238410 http://dx.doi.org/10.3390/life10110302 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Scutari, Rossana
Piermatteo, Lorenzo
Manuelli, Matteo Ciancio
Iannetta, Marco
Salpini, Romina
Bertoli, Ada
Alteri, Claudia
Saccomandi, Patrizia
Bellocchi, Maria Concetta
Malagnino, Vincenzo
Teti, Elisabetta
Sforza, Daniele
Siragusa, Leandro
Grande, Michele
Sarmati, Loredana
Svicher, Valentina
Andreoni, Massimo
Ceccherini-Silberstein, Francesca
Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
title Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
title_full Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
title_fullStr Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
title_full_unstemmed Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
title_short Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
title_sort long-term sars-cov-2 infection associated with viral dissemination in different body fluids including bile in two patients with acute cholecystitis
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700357/
https://www.ncbi.nlm.nih.gov/pubmed/33238410
http://dx.doi.org/10.3390/life10110302
work_keys_str_mv AT scutarirossana longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT piermatteolorenzo longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT manuellimatteociancio longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT iannettamarco longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT salpiniromina longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT bertoliada longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT altericlaudia longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT saccomandipatrizia longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT bellocchimariaconcetta longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT malagninovincenzo longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT tetielisabetta longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT sforzadaniele longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT siragusaleandro longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT grandemichele longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT sarmatiloredana longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT svichervalentina longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT andreonimassimo longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis
AT ceccherinisilbersteinfrancesca longtermsarscov2infectionassociatedwithviraldisseminationindifferentbodyfluidsincludingbileintwopatientswithacutecholecystitis