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Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were c...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700357/ https://www.ncbi.nlm.nih.gov/pubmed/33238410 http://dx.doi.org/10.3390/life10110302 |
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author | Scutari, Rossana Piermatteo, Lorenzo Manuelli, Matteo Ciancio Iannetta, Marco Salpini, Romina Bertoli, Ada Alteri, Claudia Saccomandi, Patrizia Bellocchi, Maria Concetta Malagnino, Vincenzo Teti, Elisabetta Sforza, Daniele Siragusa, Leandro Grande, Michele Sarmati, Loredana Svicher, Valentina Andreoni, Massimo Ceccherini-Silberstein, Francesca |
author_facet | Scutari, Rossana Piermatteo, Lorenzo Manuelli, Matteo Ciancio Iannetta, Marco Salpini, Romina Bertoli, Ada Alteri, Claudia Saccomandi, Patrizia Bellocchi, Maria Concetta Malagnino, Vincenzo Teti, Elisabetta Sforza, Daniele Siragusa, Leandro Grande, Michele Sarmati, Loredana Svicher, Valentina Andreoni, Massimo Ceccherini-Silberstein, Francesca |
author_sort | Scutari, Rossana |
collection | PubMed |
description | Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations. |
format | Online Article Text |
id | pubmed-7700357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77003572020-11-30 Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis Scutari, Rossana Piermatteo, Lorenzo Manuelli, Matteo Ciancio Iannetta, Marco Salpini, Romina Bertoli, Ada Alteri, Claudia Saccomandi, Patrizia Bellocchi, Maria Concetta Malagnino, Vincenzo Teti, Elisabetta Sforza, Daniele Siragusa, Leandro Grande, Michele Sarmati, Loredana Svicher, Valentina Andreoni, Massimo Ceccherini-Silberstein, Francesca Life (Basel) Case Report Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations. MDPI 2020-11-23 /pmc/articles/PMC7700357/ /pubmed/33238410 http://dx.doi.org/10.3390/life10110302 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Case Report Scutari, Rossana Piermatteo, Lorenzo Manuelli, Matteo Ciancio Iannetta, Marco Salpini, Romina Bertoli, Ada Alteri, Claudia Saccomandi, Patrizia Bellocchi, Maria Concetta Malagnino, Vincenzo Teti, Elisabetta Sforza, Daniele Siragusa, Leandro Grande, Michele Sarmati, Loredana Svicher, Valentina Andreoni, Massimo Ceccherini-Silberstein, Francesca Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
title | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
title_full | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
title_fullStr | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
title_full_unstemmed | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
title_short | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
title_sort | long-term sars-cov-2 infection associated with viral dissemination in different body fluids including bile in two patients with acute cholecystitis |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700357/ https://www.ncbi.nlm.nih.gov/pubmed/33238410 http://dx.doi.org/10.3390/life10110302 |
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