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Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy

Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adh...

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Autores principales: Herdade, Ana Silva, Silva, Iara Mota, Calado, Ângelo, Saldanha, Carlota, Nguyen, Ngan-Ha, Hou, Isabella, Castanho, Miguel, Roy, Sayon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700516/
https://www.ncbi.nlm.nih.gov/pubmed/33233433
http://dx.doi.org/10.3390/biom10111583
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author Herdade, Ana Silva
Silva, Iara Mota
Calado, Ângelo
Saldanha, Carlota
Nguyen, Ngan-Ha
Hou, Isabella
Castanho, Miguel
Roy, Sayon
author_facet Herdade, Ana Silva
Silva, Iara Mota
Calado, Ângelo
Saldanha, Carlota
Nguyen, Ngan-Ha
Hou, Isabella
Castanho, Miguel
Roy, Sayon
author_sort Herdade, Ana Silva
collection PubMed
description Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR.
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spelling pubmed-77005162020-11-30 Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy Herdade, Ana Silva Silva, Iara Mota Calado, Ângelo Saldanha, Carlota Nguyen, Ngan-Ha Hou, Isabella Castanho, Miguel Roy, Sayon Biomolecules Article Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR. MDPI 2020-11-21 /pmc/articles/PMC7700516/ /pubmed/33233433 http://dx.doi.org/10.3390/biom10111583 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Herdade, Ana Silva
Silva, Iara Mota
Calado, Ângelo
Saldanha, Carlota
Nguyen, Ngan-Ha
Hou, Isabella
Castanho, Miguel
Roy, Sayon
Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
title Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
title_full Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
title_fullStr Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
title_full_unstemmed Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
title_short Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
title_sort effects of diabetes on microcirculation and leukostasis in retinal and non-ocular tissues: implications for diabetic retinopathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700516/
https://www.ncbi.nlm.nih.gov/pubmed/33233433
http://dx.doi.org/10.3390/biom10111583
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