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Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method

The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and ro...

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Detalles Bibliográficos
Autores principales: Ziolkowska, Anna, Debska, Bozena, Banach-Szott, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700638/
https://www.ncbi.nlm.nih.gov/pubmed/33266357
http://dx.doi.org/10.3390/molecules25225462
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author Ziolkowska, Anna
Debska, Bozena
Banach-Szott, Magdalena
author_facet Ziolkowska, Anna
Debska, Bozena
Banach-Szott, Magdalena
author_sort Ziolkowska, Anna
collection PubMed
description The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and roots) and soil—Albic Brunic Arenosol (horizon A, AE, and Bsv) samples. Phenolic compounds were isolated at two stages by applying acid hydrolysis followed by alkaline re-hydrolysis. The quantitative and qualitative analysis of phenolic compounds was performed with high-performance liquid chromatography with a DAD. The content of phenolic compounds in the extracts depended on the hydrolysis method and it was determined by the type of the research material. The amounts of phenolic compounds contained in the alkaline hydrolysates accounted for 55.7% (soil, horizon Bsv)—454% (roots) of their content in acid hydrolysates. In the extracts from acid hydrolysates, chlorogenic and p-hydroxybenzoic acids were dominant. In the alkaline extracts from the plant material, the highest content was recorded for p-coumaric and ferulic acids, and in the extracts from soil, ferulic and chlorogenic acids. A combination of acid and alkaline hydrolysis ensures the best extraction efficiency of insoluble-bound forms of polyphenols from plant and soil material.
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spelling pubmed-77006382020-11-30 Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method Ziolkowska, Anna Debska, Bozena Banach-Szott, Magdalena Molecules Article The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and roots) and soil—Albic Brunic Arenosol (horizon A, AE, and Bsv) samples. Phenolic compounds were isolated at two stages by applying acid hydrolysis followed by alkaline re-hydrolysis. The quantitative and qualitative analysis of phenolic compounds was performed with high-performance liquid chromatography with a DAD. The content of phenolic compounds in the extracts depended on the hydrolysis method and it was determined by the type of the research material. The amounts of phenolic compounds contained in the alkaline hydrolysates accounted for 55.7% (soil, horizon Bsv)—454% (roots) of their content in acid hydrolysates. In the extracts from acid hydrolysates, chlorogenic and p-hydroxybenzoic acids were dominant. In the alkaline extracts from the plant material, the highest content was recorded for p-coumaric and ferulic acids, and in the extracts from soil, ferulic and chlorogenic acids. A combination of acid and alkaline hydrolysis ensures the best extraction efficiency of insoluble-bound forms of polyphenols from plant and soil material. MDPI 2020-11-22 /pmc/articles/PMC7700638/ /pubmed/33266357 http://dx.doi.org/10.3390/molecules25225462 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ziolkowska, Anna
Debska, Bozena
Banach-Szott, Magdalena
Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
title Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
title_full Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
title_fullStr Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
title_full_unstemmed Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
title_short Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
title_sort content of phenolic compounds in meadow vegetation and soil depending on the isolation method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700638/
https://www.ncbi.nlm.nih.gov/pubmed/33266357
http://dx.doi.org/10.3390/molecules25225462
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