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Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota

Galacto-oligosaccharide (GOS) has been added to infant formula as prebiotics and can bring many benefits to human health. This study proved the effect of GOS in prevention and alleviation against E. coli O157 invasion and colonization and the mechanism behind this was explored in a mice model. The r...

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Detalles Bibliográficos
Autores principales: Zou, Yan, Wang, Jin, Wang, Yuanyifei, Peng, Bo, Liu, Jingmin, Zhang, Bowei, Lv, Huan, Wang, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700679/
https://www.ncbi.nlm.nih.gov/pubmed/33233359
http://dx.doi.org/10.3390/foods9111710
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author Zou, Yan
Wang, Jin
Wang, Yuanyifei
Peng, Bo
Liu, Jingmin
Zhang, Bowei
Lv, Huan
Wang, Shuo
author_facet Zou, Yan
Wang, Jin
Wang, Yuanyifei
Peng, Bo
Liu, Jingmin
Zhang, Bowei
Lv, Huan
Wang, Shuo
author_sort Zou, Yan
collection PubMed
description Galacto-oligosaccharide (GOS) has been added to infant formula as prebiotics and can bring many benefits to human health. This study proved the effect of GOS in prevention and alleviation against E. coli O157 invasion and colonization and the mechanism behind this was explored in a mice model. The results showed that the expression of Muc2 and Occlaudin were both significantly down-regulated (p < 0.05) by E. coli O157 infection, while GOS alleviated this phenomenon, which means that GOS can reduce the colonization of E. coli O157 by enhancing the gut barrier function. Through the determination of inflammatory cytokines, we found that GOS can relieve inflammation caused by pathogens. At the same time, GOS can promote the growth of probiotics such as Akkermansia, Ruminococcaceae and Bacteroides, thus modulating microorganism environments and improving short chain fatty acid (SCFA) levels in the intestine. This study provides an explanation for the mechanism behind the protection of GOS against pathogen infection.
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spelling pubmed-77006792020-11-30 Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota Zou, Yan Wang, Jin Wang, Yuanyifei Peng, Bo Liu, Jingmin Zhang, Bowei Lv, Huan Wang, Shuo Foods Article Galacto-oligosaccharide (GOS) has been added to infant formula as prebiotics and can bring many benefits to human health. This study proved the effect of GOS in prevention and alleviation against E. coli O157 invasion and colonization and the mechanism behind this was explored in a mice model. The results showed that the expression of Muc2 and Occlaudin were both significantly down-regulated (p < 0.05) by E. coli O157 infection, while GOS alleviated this phenomenon, which means that GOS can reduce the colonization of E. coli O157 by enhancing the gut barrier function. Through the determination of inflammatory cytokines, we found that GOS can relieve inflammation caused by pathogens. At the same time, GOS can promote the growth of probiotics such as Akkermansia, Ruminococcaceae and Bacteroides, thus modulating microorganism environments and improving short chain fatty acid (SCFA) levels in the intestine. This study provides an explanation for the mechanism behind the protection of GOS against pathogen infection. MDPI 2020-11-21 /pmc/articles/PMC7700679/ /pubmed/33233359 http://dx.doi.org/10.3390/foods9111710 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zou, Yan
Wang, Jin
Wang, Yuanyifei
Peng, Bo
Liu, Jingmin
Zhang, Bowei
Lv, Huan
Wang, Shuo
Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota
title Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota
title_full Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota
title_fullStr Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota
title_full_unstemmed Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota
title_short Protection of Galacto-Oligosaccharide against E. coli O157 Colonization through Enhancing Gut Barrier Function and Modulating Gut Microbiota
title_sort protection of galacto-oligosaccharide against e. coli o157 colonization through enhancing gut barrier function and modulating gut microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700679/
https://www.ncbi.nlm.nih.gov/pubmed/33233359
http://dx.doi.org/10.3390/foods9111710
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