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Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Molecular and Cellular Biology
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700842/ https://www.ncbi.nlm.nih.gov/pubmed/33199671 http://dx.doi.org/10.14348/molcells.2020.0177 |
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author | Kim, Woo Ryung Park, Eun Gyung Kang, Kyung-Won Lee, Sang-Myeong Kim, Bumseok Kim, Heui-Soo |
author_facet | Kim, Woo Ryung Park, Eun Gyung Kang, Kyung-Won Lee, Sang-Myeong Kim, Bumseok Kim, Heui-Soo |
author_sort | Kim, Woo Ryung |
collection | PubMed |
description | Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-7700842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Molecular and Cellular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-77008422020-12-08 Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 Kim, Woo Ryung Park, Eun Gyung Kang, Kyung-Won Lee, Sang-Myeong Kim, Bumseok Kim, Heui-Soo Mol Cells Research Article Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection. Korean Society for Molecular and Cellular Biology 2020-11-30 2020-11-15 /pmc/articles/PMC7700842/ /pubmed/33199671 http://dx.doi.org/10.14348/molcells.2020.0177 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ (https://creativecommons.org/licenses/by-nc-sa/3.0/) |
spellingShingle | Research Article Kim, Woo Ryung Park, Eun Gyung Kang, Kyung-Won Lee, Sang-Myeong Kim, Bumseok Kim, Heui-Soo Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 |
title | Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 |
title_full | Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 |
title_fullStr | Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 |
title_full_unstemmed | Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 |
title_short | Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 |
title_sort | expression analyses of micrornas in hamster lung tissues infected by sars-cov-2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700842/ https://www.ncbi.nlm.nih.gov/pubmed/33199671 http://dx.doi.org/10.14348/molcells.2020.0177 |
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