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Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe...

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Autores principales: Kim, Woo Ryung, Park, Eun Gyung, Kang, Kyung-Won, Lee, Sang-Myeong, Kim, Bumseok, Kim, Heui-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700842/
https://www.ncbi.nlm.nih.gov/pubmed/33199671
http://dx.doi.org/10.14348/molcells.2020.0177
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author Kim, Woo Ryung
Park, Eun Gyung
Kang, Kyung-Won
Lee, Sang-Myeong
Kim, Bumseok
Kim, Heui-Soo
author_facet Kim, Woo Ryung
Park, Eun Gyung
Kang, Kyung-Won
Lee, Sang-Myeong
Kim, Bumseok
Kim, Heui-Soo
author_sort Kim, Woo Ryung
collection PubMed
description Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.
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spelling pubmed-77008422020-12-08 Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2 Kim, Woo Ryung Park, Eun Gyung Kang, Kyung-Won Lee, Sang-Myeong Kim, Bumseok Kim, Heui-Soo Mol Cells Research Article Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection. Korean Society for Molecular and Cellular Biology 2020-11-30 2020-11-15 /pmc/articles/PMC7700842/ /pubmed/33199671 http://dx.doi.org/10.14348/molcells.2020.0177 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ (https://creativecommons.org/licenses/by-nc-sa/3.0/)
spellingShingle Research Article
Kim, Woo Ryung
Park, Eun Gyung
Kang, Kyung-Won
Lee, Sang-Myeong
Kim, Bumseok
Kim, Heui-Soo
Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
title Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
title_full Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
title_fullStr Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
title_full_unstemmed Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
title_short Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2
title_sort expression analyses of micrornas in hamster lung tissues infected by sars-cov-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700842/
https://www.ncbi.nlm.nih.gov/pubmed/33199671
http://dx.doi.org/10.14348/molcells.2020.0177
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