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High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry

Blueberry (Vaccinium corymbosum and hybrids) is an autotetraploid crop whose commercial relevance has been growing steadily during the last 20 years. However, the ever-increasing cost of labor for hand-picking blueberry is one main constraint in competitive marketing of the fruit. Machine harvestabi...

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Autores principales: Cappai, Francesco, Amadeu, Rodrigo R., Benevenuto, Juliana, Cullen, Ryan, Garcia, Alexandria, Grossman, Adina, Ferrão, Luís Felipe V., Munoz, Patricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701094/
https://www.ncbi.nlm.nih.gov/pubmed/33304360
http://dx.doi.org/10.3389/fpls.2020.562171
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author Cappai, Francesco
Amadeu, Rodrigo R.
Benevenuto, Juliana
Cullen, Ryan
Garcia, Alexandria
Grossman, Adina
Ferrão, Luís Felipe V.
Munoz, Patricio
author_facet Cappai, Francesco
Amadeu, Rodrigo R.
Benevenuto, Juliana
Cullen, Ryan
Garcia, Alexandria
Grossman, Adina
Ferrão, Luís Felipe V.
Munoz, Patricio
author_sort Cappai, Francesco
collection PubMed
description Blueberry (Vaccinium corymbosum and hybrids) is an autotetraploid crop whose commercial relevance has been growing steadily during the last 20 years. However, the ever-increasing cost of labor for hand-picking blueberry is one main constraint in competitive marketing of the fruit. Machine harvestability is, therefore, a key trait for the blueberry industry. Understanding the genetic architecture of traits related to machine harvestability through Quantitative Trait Loci (QTL) mapping is the first step toward implementation of molecular breeding for faster genetic gains. Despite recent advances in software development for autotetraploid genetic mapping, a high-resolution map is still not available for blueberry. In this study, we crafted a map for autotetraploid low-chill highbush blueberry containing 11,292 SNP markers and a total size of 1,953.97 cM (average density of 5.78 markers/cM). This map was subsequently used to perform QTL analyses in 2-year field trials for a trait crucial to machine harvesting: fruit firmness. Preliminary insights were also sought for single evaluations of firmness retention after cold storage, and fruit detachment force traits. Significant QTL peaks were identified for all the traits and overlapping QTL intervals were detected for firmness across the years. We found low-to-moderate QTL effects explaining the phenotypic variance, which suggest a quantitative nature of these traits. The QTL intervals were further speculated for putative gene repertoire. Altogether, our findings provide the basis for future fine-mapping and molecular breeding efforts for machine harvesting in blueberry.
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spelling pubmed-77010942020-12-09 High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry Cappai, Francesco Amadeu, Rodrigo R. Benevenuto, Juliana Cullen, Ryan Garcia, Alexandria Grossman, Adina Ferrão, Luís Felipe V. Munoz, Patricio Front Plant Sci Plant Science Blueberry (Vaccinium corymbosum and hybrids) is an autotetraploid crop whose commercial relevance has been growing steadily during the last 20 years. However, the ever-increasing cost of labor for hand-picking blueberry is one main constraint in competitive marketing of the fruit. Machine harvestability is, therefore, a key trait for the blueberry industry. Understanding the genetic architecture of traits related to machine harvestability through Quantitative Trait Loci (QTL) mapping is the first step toward implementation of molecular breeding for faster genetic gains. Despite recent advances in software development for autotetraploid genetic mapping, a high-resolution map is still not available for blueberry. In this study, we crafted a map for autotetraploid low-chill highbush blueberry containing 11,292 SNP markers and a total size of 1,953.97 cM (average density of 5.78 markers/cM). This map was subsequently used to perform QTL analyses in 2-year field trials for a trait crucial to machine harvesting: fruit firmness. Preliminary insights were also sought for single evaluations of firmness retention after cold storage, and fruit detachment force traits. Significant QTL peaks were identified for all the traits and overlapping QTL intervals were detected for firmness across the years. We found low-to-moderate QTL effects explaining the phenotypic variance, which suggest a quantitative nature of these traits. The QTL intervals were further speculated for putative gene repertoire. Altogether, our findings provide the basis for future fine-mapping and molecular breeding efforts for machine harvesting in blueberry. Frontiers Media S.A. 2020-11-16 /pmc/articles/PMC7701094/ /pubmed/33304360 http://dx.doi.org/10.3389/fpls.2020.562171 Text en Copyright © 2020 Cappai, Amadeu, Benevenuto, Cullen, Garcia, Grossman, Ferrão and Munoz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cappai, Francesco
Amadeu, Rodrigo R.
Benevenuto, Juliana
Cullen, Ryan
Garcia, Alexandria
Grossman, Adina
Ferrão, Luís Felipe V.
Munoz, Patricio
High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry
title High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry
title_full High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry
title_fullStr High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry
title_full_unstemmed High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry
title_short High-Resolution Linkage Map and QTL Analyses of Fruit Firmness in Autotetraploid Blueberry
title_sort high-resolution linkage map and qtl analyses of fruit firmness in autotetraploid blueberry
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701094/
https://www.ncbi.nlm.nih.gov/pubmed/33304360
http://dx.doi.org/10.3389/fpls.2020.562171
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