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Effects of Chemical and Mechanical Lysis on Microbial DNA Yield, Integrity, and Downstream Amplicon Sequencing of Rumen Bacteria and Protozoa

QIAamp Fast DNA Stool Mini Kit (QIAGEN, Valencia, CA, United States) and RBB + C (Yu and Morrison, 2004) methodologies are widely employed to extract microbial DNA from rumen samples and can exhibit different efficiencies of obtaining DNA yield, quality, and downstream amplicon sequence analysis. No...

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Detalles Bibliográficos
Autores principales:	Ma, Zhi Yuan, Zhang, Xiu Min, Wang, Rong, Wang, Min, Liu, Ting, Tan, Zhi Liang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701101/ https://www.ncbi.nlm.nih.gov/pubmed/33304329 http://dx.doi.org/10.3389/fmicb.2020.581227

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