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IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs
The olfactory organs (OOs) of vertebrates play important roles in their extraordinary chemosensory capacity, a process during which they are continuously exposed to environmental pathogens. Nasopharynx-associated lymphoid tissue (NALT) contains B cells and immunoglobulins (Igs), which function as th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701277/ https://www.ncbi.nlm.nih.gov/pubmed/33304348 http://dx.doi.org/10.3389/fimmu.2020.583740 |
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author | Dong, Fen Yin, Guang-mei Meng, Kai-feng Xu, Hao-yue Liu, Xia Wang, Qing-chao Xu, Zhen |
author_facet | Dong, Fen Yin, Guang-mei Meng, Kai-feng Xu, Hao-yue Liu, Xia Wang, Qing-chao Xu, Zhen |
author_sort | Dong, Fen |
collection | PubMed |
description | The olfactory organs (OOs) of vertebrates play important roles in their extraordinary chemosensory capacity, a process during which they are continuously exposed to environmental pathogens. Nasopharynx-associated lymphoid tissue (NALT) contains B cells and immunoglobulins (Igs), which function as the first defense line against antigens in mammals and also exist in teleosts. However, the immune responses of teleost NALT B cells and Igs during bacterial infection remain largely uncharacterized. In this study, rainbow trout were infected with Flavobacterium columnare via continuous immersion, after which the adaptive immune responses within NALT were evaluated. F. columnare could invade trout nasal mucosa and cause histopathological changes in trout OO. Moreover, the accumulation of IgT(+) B cells in trout nasal mucosa was induced by bacterial challenge, which was accompanied by strong bacteria-specific IgT responses in the nasal mucus. Importantly, our study is the first to report local nasal-specific immune responses in teleosts during bacterial challenge by characterizing the local proliferation of IgT(+) B cells and generation of bacteria-specific IgT in trout OOs after F. columnare infection. In addition to the strong IgT and IgT(+) B cells responses in OO, bacteria-specific IgT and IgM were also detected in serum following bacterial challenge. Taken together, our findings suggest that IgT functions as an important mucosal Ig in teleost NALT and mediates local adaptive immunity during bacterial infection, which is similar to their protective role during parasitic infection. |
format | Online Article Text |
id | pubmed-7701277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77012772020-12-09 IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs Dong, Fen Yin, Guang-mei Meng, Kai-feng Xu, Hao-yue Liu, Xia Wang, Qing-chao Xu, Zhen Front Immunol Immunology The olfactory organs (OOs) of vertebrates play important roles in their extraordinary chemosensory capacity, a process during which they are continuously exposed to environmental pathogens. Nasopharynx-associated lymphoid tissue (NALT) contains B cells and immunoglobulins (Igs), which function as the first defense line against antigens in mammals and also exist in teleosts. However, the immune responses of teleost NALT B cells and Igs during bacterial infection remain largely uncharacterized. In this study, rainbow trout were infected with Flavobacterium columnare via continuous immersion, after which the adaptive immune responses within NALT were evaluated. F. columnare could invade trout nasal mucosa and cause histopathological changes in trout OO. Moreover, the accumulation of IgT(+) B cells in trout nasal mucosa was induced by bacterial challenge, which was accompanied by strong bacteria-specific IgT responses in the nasal mucus. Importantly, our study is the first to report local nasal-specific immune responses in teleosts during bacterial challenge by characterizing the local proliferation of IgT(+) B cells and generation of bacteria-specific IgT in trout OOs after F. columnare infection. In addition to the strong IgT and IgT(+) B cells responses in OO, bacteria-specific IgT and IgM were also detected in serum following bacterial challenge. Taken together, our findings suggest that IgT functions as an important mucosal Ig in teleost NALT and mediates local adaptive immunity during bacterial infection, which is similar to their protective role during parasitic infection. Frontiers Media S.A. 2020-11-16 /pmc/articles/PMC7701277/ /pubmed/33304348 http://dx.doi.org/10.3389/fimmu.2020.583740 Text en Copyright © 2020 Dong, Yin, Meng, Xu, Liu, Wang and Xu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Dong, Fen Yin, Guang-mei Meng, Kai-feng Xu, Hao-yue Liu, Xia Wang, Qing-chao Xu, Zhen IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs |
title | IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs |
title_full | IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs |
title_fullStr | IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs |
title_full_unstemmed | IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs |
title_short | IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs |
title_sort | igt plays a predominant role in the antibacterial immunity of rainbow trout olfactory organs |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701277/ https://www.ncbi.nlm.nih.gov/pubmed/33304348 http://dx.doi.org/10.3389/fimmu.2020.583740 |
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