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DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells

DEP domain containing 1(DEPDC1) is involved in the tumorigenesis of a variety of cancers. But its role in tumorigenesis of lung adenocarcinoma (LUAD) is not fully understood. Here, we investigated the role and the underlying mechanisms of DEPDC1 in the development of LUAD. The expression and prognos...

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Autores principales: Wang, Wei, Li, Aili, Han, Xiaodan, Wang, Qingqing, Guo, Jinyong, Wu, Youru, Wang, Chen, Huang, Guojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701574/
https://www.ncbi.nlm.nih.gov/pubmed/33021072
http://dx.doi.org/10.1111/jcmm.15947
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author Wang, Wei
Li, Aili
Han, Xiaodan
Wang, Qingqing
Guo, Jinyong
Wu, Youru
Wang, Chen
Huang, Guojin
author_facet Wang, Wei
Li, Aili
Han, Xiaodan
Wang, Qingqing
Guo, Jinyong
Wu, Youru
Wang, Chen
Huang, Guojin
author_sort Wang, Wei
collection PubMed
description DEP domain containing 1(DEPDC1) is involved in the tumorigenesis of a variety of cancers. But its role in tumorigenesis of lung adenocarcinoma (LUAD) is not fully understood. Here, we investigated the role and the underlying mechanisms of DEPDC1 in the development of LUAD. The expression and prognostic values of DEPDC1 in LUAD were analysed by using the data from public databases. Gene enrichment in TCGA LUAD was analysed using GSEA software with the pre‐defined gene sets. Cell proliferation, migration and invasion of A549 cells were examined with colony formation, Transwell and wound healing assays. The function of DEPDC1 in autophagy and RAS‐ERK1/2 signalling was determined with Western blot assay upon DEPDC1 knockdown and/or overexpression in A549, HCC827 and H1993 cells. The results demonstrated that DEPDC1 expression was up‐regulated in LUAD tissues, and its high expression was correlated with unfavourable prognosis. The data also showed that DEPDC1 knockdown impaired proliferation, migration and invasion of A549 cells. Most notably, the results showed that DEPDC1 up‐regulated RAS expression and thus enhanced ERK1/2 activity, through which DEPDC1 could inhibit autophagy. In conclusion, our study revealed that DEPDC1 is up‐regulated in LUAD tissues and plays an oncogenic role in LUAD, and that DEPDC1 inhibits autophagy through the RAS‐ERK1/2 signalling in A549, HCC827 and H1993 cells.
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spelling pubmed-77015742020-12-08 DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells Wang, Wei Li, Aili Han, Xiaodan Wang, Qingqing Guo, Jinyong Wu, Youru Wang, Chen Huang, Guojin J Cell Mol Med Original Articles DEP domain containing 1(DEPDC1) is involved in the tumorigenesis of a variety of cancers. But its role in tumorigenesis of lung adenocarcinoma (LUAD) is not fully understood. Here, we investigated the role and the underlying mechanisms of DEPDC1 in the development of LUAD. The expression and prognostic values of DEPDC1 in LUAD were analysed by using the data from public databases. Gene enrichment in TCGA LUAD was analysed using GSEA software with the pre‐defined gene sets. Cell proliferation, migration and invasion of A549 cells were examined with colony formation, Transwell and wound healing assays. The function of DEPDC1 in autophagy and RAS‐ERK1/2 signalling was determined with Western blot assay upon DEPDC1 knockdown and/or overexpression in A549, HCC827 and H1993 cells. The results demonstrated that DEPDC1 expression was up‐regulated in LUAD tissues, and its high expression was correlated with unfavourable prognosis. The data also showed that DEPDC1 knockdown impaired proliferation, migration and invasion of A549 cells. Most notably, the results showed that DEPDC1 up‐regulated RAS expression and thus enhanced ERK1/2 activity, through which DEPDC1 could inhibit autophagy. In conclusion, our study revealed that DEPDC1 is up‐regulated in LUAD tissues and plays an oncogenic role in LUAD, and that DEPDC1 inhibits autophagy through the RAS‐ERK1/2 signalling in A549, HCC827 and H1993 cells. John Wiley and Sons Inc. 2020-10-05 2020-11 /pmc/articles/PMC7701574/ /pubmed/33021072 http://dx.doi.org/10.1111/jcmm.15947 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Wei
Li, Aili
Han, Xiaodan
Wang, Qingqing
Guo, Jinyong
Wu, Youru
Wang, Chen
Huang, Guojin
DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells
title DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells
title_full DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells
title_fullStr DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells
title_full_unstemmed DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells
title_short DEPDC1 up‐regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells
title_sort depdc1 up‐regulates ras expression to inhibit autophagy in lung adenocarcinoma cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701574/
https://www.ncbi.nlm.nih.gov/pubmed/33021072
http://dx.doi.org/10.1111/jcmm.15947
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