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RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture

It is well known that dermal papilla cells (DPCs) are crucial for hair follicle growth and regeneration. However, dermal papilla cells in 2D culture could lose their ability of regeneration after several passage intervals. As opposed to DPCs in 2D culture, the DPCs in 3D culture could passage extens...

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Autores principales: Lin, Guanyu, Yin, Guoqian, Ye, Jun, Pan, Xinyuan, Zhu, Jiangying, Lin, Bojie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701577/
https://www.ncbi.nlm.nih.gov/pubmed/33038058
http://dx.doi.org/10.1111/jcmm.15965
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author Lin, Guanyu
Yin, Guoqian
Ye, Jun
Pan, Xinyuan
Zhu, Jiangying
Lin, Bojie
author_facet Lin, Guanyu
Yin, Guoqian
Ye, Jun
Pan, Xinyuan
Zhu, Jiangying
Lin, Bojie
author_sort Lin, Guanyu
collection PubMed
description It is well known that dermal papilla cells (DPCs) are crucial for hair follicle growth and regeneration. However, dermal papilla cells in 2D culture could lose their ability of regeneration after several passage intervals. As opposed to DPCs in 2D culture, the DPCs in 3D culture could passage extensively. However, the molecular mechanisms of DPCs’ regeneration in 3D culture remain unclear. Accordingly, gene sequencing is recommended for the investigation of hair regeneration between 2D and 3D culture, the three groups were established including DPCs in passage 2 in 2D culture, DPCs in passage 8 in 2D culture and DPCs in passage 8 in 3D culture. The differentially expressed genes (DEGs) were identified using the Venn diagram of these three groups, which included 1642 known and 359 novel genes, respectively. A total of 1642 known genes were used for Gene Ontology (GO), Kyoto Gene, Genomic Encyclopedia (KEGG) pathway enrichment and protein‐protein interaction (PPI) analyses, respectively. The functions and pathways of DEGs were enriched in biological regulation, signal transduction and immune system, etc. The key module and the top 10 hub genes (IL1B, CXCL12, HGF, EGFR, APP, CCL2, PTGS2, MMP9, NGF and SPP1) were also identified using the Cytoscape application. Furthermore, the qRT‐PCR results of the three groups validated that the hub genes were crucial for hair growth. In conclusion, the ten identified hub genes and related pathways in the current study can be used to understand the molecular mechanism of hair growth, and those provided a possibility for hair regeneration.
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spelling pubmed-77015772020-12-08 RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture Lin, Guanyu Yin, Guoqian Ye, Jun Pan, Xinyuan Zhu, Jiangying Lin, Bojie J Cell Mol Med Original Articles It is well known that dermal papilla cells (DPCs) are crucial for hair follicle growth and regeneration. However, dermal papilla cells in 2D culture could lose their ability of regeneration after several passage intervals. As opposed to DPCs in 2D culture, the DPCs in 3D culture could passage extensively. However, the molecular mechanisms of DPCs’ regeneration in 3D culture remain unclear. Accordingly, gene sequencing is recommended for the investigation of hair regeneration between 2D and 3D culture, the three groups were established including DPCs in passage 2 in 2D culture, DPCs in passage 8 in 2D culture and DPCs in passage 8 in 3D culture. The differentially expressed genes (DEGs) were identified using the Venn diagram of these three groups, which included 1642 known and 359 novel genes, respectively. A total of 1642 known genes were used for Gene Ontology (GO), Kyoto Gene, Genomic Encyclopedia (KEGG) pathway enrichment and protein‐protein interaction (PPI) analyses, respectively. The functions and pathways of DEGs were enriched in biological regulation, signal transduction and immune system, etc. The key module and the top 10 hub genes (IL1B, CXCL12, HGF, EGFR, APP, CCL2, PTGS2, MMP9, NGF and SPP1) were also identified using the Cytoscape application. Furthermore, the qRT‐PCR results of the three groups validated that the hub genes were crucial for hair growth. In conclusion, the ten identified hub genes and related pathways in the current study can be used to understand the molecular mechanism of hair growth, and those provided a possibility for hair regeneration. John Wiley and Sons Inc. 2020-10-10 2020-11 /pmc/articles/PMC7701577/ /pubmed/33038058 http://dx.doi.org/10.1111/jcmm.15965 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Lin, Guanyu
Yin, Guoqian
Ye, Jun
Pan, Xinyuan
Zhu, Jiangying
Lin, Bojie
RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
title RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
title_full RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
title_fullStr RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
title_full_unstemmed RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
title_short RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture
title_sort rna sequence analysis of dermal papilla cells’ regeneration in 3d culture
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701577/
https://www.ncbi.nlm.nih.gov/pubmed/33038058
http://dx.doi.org/10.1111/jcmm.15965
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