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Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model
Oncolytic adenovirus (OA) has attracted increasing attention due to their specific proliferation in tumour cells and resulting in lysis of tumour cells. To further improve the antitumour effect of OA, in this study, we combined CD55‐TRAIL‐IETD‐MnSOD (CD55‐TMn), a CEA‐controlled OA constructed previo...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701578/ https://www.ncbi.nlm.nih.gov/pubmed/33251723 http://dx.doi.org/10.1111/jcmm.15966 |
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author | Xiao, Boduan Ying, Chang Chen, Yongyi Huang, Fang Wang, Binrong Fang, Huiling Guo, Wan Liu, Tao Zhou, Xiumei Huang, Biao Liu, Xinyuan Wang, Yigang |
author_facet | Xiao, Boduan Ying, Chang Chen, Yongyi Huang, Fang Wang, Binrong Fang, Huiling Guo, Wan Liu, Tao Zhou, Xiumei Huang, Biao Liu, Xinyuan Wang, Yigang |
author_sort | Xiao, Boduan |
collection | PubMed |
description | Oncolytic adenovirus (OA) has attracted increasing attention due to their specific proliferation in tumour cells and resulting in lysis of tumour cells. To further improve the antitumour effect of OA, in this study, we combined CD55‐TRAIL‐IETD‐MnSOD (CD55‐TMn), a CEA‐controlled OA constructed previously, and chemotherapy to investigate their synergistic effect and possible mechanisms. MTT assay was performed to detect antitumour effects. Hoechst 33 342 and flow cytometric analysis were used to examine cell apoptosis. Western blotting was performed to examine cell pyroptosis and apoptosis mechanism. Animal experiment was used to detect antitumour effect of doxorubicin hydrochloride (Dox) combined with CD55‐TMn in vivo. We firstly found that Dox promotes gene expression mediated by CEA‐regulated OA and virus progeny replication by activating phosphorylation of Smad3, and Dox can enhance antitumour effect of CEA‐regulated CD55‐TMn by promoting cell apotopsis and cell pyroptosis. Thus, our results provide an experimental and theoretical basis on tumour therapy by combination treatment of the oncolytic virotherapy and chemotherapy and it is expected to become a novel strategy for liver cancer therapy. |
format | Online Article Text |
id | pubmed-7701578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77015782020-12-08 Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model Xiao, Boduan Ying, Chang Chen, Yongyi Huang, Fang Wang, Binrong Fang, Huiling Guo, Wan Liu, Tao Zhou, Xiumei Huang, Biao Liu, Xinyuan Wang, Yigang J Cell Mol Med Original Articles Oncolytic adenovirus (OA) has attracted increasing attention due to their specific proliferation in tumour cells and resulting in lysis of tumour cells. To further improve the antitumour effect of OA, in this study, we combined CD55‐TRAIL‐IETD‐MnSOD (CD55‐TMn), a CEA‐controlled OA constructed previously, and chemotherapy to investigate their synergistic effect and possible mechanisms. MTT assay was performed to detect antitumour effects. Hoechst 33 342 and flow cytometric analysis were used to examine cell apoptosis. Western blotting was performed to examine cell pyroptosis and apoptosis mechanism. Animal experiment was used to detect antitumour effect of doxorubicin hydrochloride (Dox) combined with CD55‐TMn in vivo. We firstly found that Dox promotes gene expression mediated by CEA‐regulated OA and virus progeny replication by activating phosphorylation of Smad3, and Dox can enhance antitumour effect of CEA‐regulated CD55‐TMn by promoting cell apotopsis and cell pyroptosis. Thus, our results provide an experimental and theoretical basis on tumour therapy by combination treatment of the oncolytic virotherapy and chemotherapy and it is expected to become a novel strategy for liver cancer therapy. John Wiley and Sons Inc. 2020-10-14 2020-11 /pmc/articles/PMC7701578/ /pubmed/33251723 http://dx.doi.org/10.1111/jcmm.15966 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Xiao, Boduan Ying, Chang Chen, Yongyi Huang, Fang Wang, Binrong Fang, Huiling Guo, Wan Liu, Tao Zhou, Xiumei Huang, Biao Liu, Xinyuan Wang, Yigang Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
title | Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
title_full | Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
title_fullStr | Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
title_full_unstemmed | Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
title_short | Doxorubicin hydrochloride enhanced antitumour effect of CEA‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
title_sort | doxorubicin hydrochloride enhanced antitumour effect of cea‐regulated oncolytic virotherapy in live cancer cells and a mouse model |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701578/ https://www.ncbi.nlm.nih.gov/pubmed/33251723 http://dx.doi.org/10.1111/jcmm.15966 |
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