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Purification and characterisation of new laccase from Trametes polyzona WRF03

The molecular screening for laccase specific gene sequences in Trametes polyzona WRF03 (TpWRF03) using designed oligonucleotide primers analogous to the conserved sequences on the copper-binding regions of known laccases showed positive amplification with an amplicon size corresponding to 1500 bp. T...

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Autores principales: Ezike, Tobechukwu Christian, Ezugwu, Arinze Linus, Udeh, Jerry Okwudili, Eze, Sabinus Oscar Onyebuchi, Chilaka, Ferdinand Chiemeka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701954/
https://www.ncbi.nlm.nih.gov/pubmed/33299811
http://dx.doi.org/10.1016/j.btre.2020.e00566
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author Ezike, Tobechukwu Christian
Ezugwu, Arinze Linus
Udeh, Jerry Okwudili
Eze, Sabinus Oscar Onyebuchi
Chilaka, Ferdinand Chiemeka
author_facet Ezike, Tobechukwu Christian
Ezugwu, Arinze Linus
Udeh, Jerry Okwudili
Eze, Sabinus Oscar Onyebuchi
Chilaka, Ferdinand Chiemeka
author_sort Ezike, Tobechukwu Christian
collection PubMed
description The molecular screening for laccase specific gene sequences in Trametes polyzona WRF03 (TpWRF03) using designed oligonucleotide primers analogous to the conserved sequences on the copper-binding regions of known laccases showed positive amplification with an amplicon size corresponding to 1500 bp. The purified TpWRF03 laccase (TpL) is a monomer with a molecular weight corresponding to 66 kDa. The enzyme had an optimal pH of 4.5 and temperature of 55 °C. TpL was most stable within pH of 5.5–6.5 and at a temperature range of 40–50 °C. Sodium azide, sodium cyanide and Fe(2+) greatly inhibited the enzyme activity. TpL showed more than 50 % decolourisation efficiency on coomassie brilliant blue (72.35 %) and malachite green (57.84 %) but displayed low decolourisation efficiency towards Azure B (1.78 %) and methylene blue (0.38 %). The results showed that TpWRF03 produces high-yield of true laccase with robust properties for biotechnological applications.
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spelling pubmed-77019542020-12-08 Purification and characterisation of new laccase from Trametes polyzona WRF03 Ezike, Tobechukwu Christian Ezugwu, Arinze Linus Udeh, Jerry Okwudili Eze, Sabinus Oscar Onyebuchi Chilaka, Ferdinand Chiemeka Biotechnol Rep (Amst) Research Article The molecular screening for laccase specific gene sequences in Trametes polyzona WRF03 (TpWRF03) using designed oligonucleotide primers analogous to the conserved sequences on the copper-binding regions of known laccases showed positive amplification with an amplicon size corresponding to 1500 bp. The purified TpWRF03 laccase (TpL) is a monomer with a molecular weight corresponding to 66 kDa. The enzyme had an optimal pH of 4.5 and temperature of 55 °C. TpL was most stable within pH of 5.5–6.5 and at a temperature range of 40–50 °C. Sodium azide, sodium cyanide and Fe(2+) greatly inhibited the enzyme activity. TpL showed more than 50 % decolourisation efficiency on coomassie brilliant blue (72.35 %) and malachite green (57.84 %) but displayed low decolourisation efficiency towards Azure B (1.78 %) and methylene blue (0.38 %). The results showed that TpWRF03 produces high-yield of true laccase with robust properties for biotechnological applications. Elsevier 2020-11-20 /pmc/articles/PMC7701954/ /pubmed/33299811 http://dx.doi.org/10.1016/j.btre.2020.e00566 Text en © 2020 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Ezike, Tobechukwu Christian
Ezugwu, Arinze Linus
Udeh, Jerry Okwudili
Eze, Sabinus Oscar Onyebuchi
Chilaka, Ferdinand Chiemeka
Purification and characterisation of new laccase from Trametes polyzona WRF03
title Purification and characterisation of new laccase from Trametes polyzona WRF03
title_full Purification and characterisation of new laccase from Trametes polyzona WRF03
title_fullStr Purification and characterisation of new laccase from Trametes polyzona WRF03
title_full_unstemmed Purification and characterisation of new laccase from Trametes polyzona WRF03
title_short Purification and characterisation of new laccase from Trametes polyzona WRF03
title_sort purification and characterisation of new laccase from trametes polyzona wrf03
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701954/
https://www.ncbi.nlm.nih.gov/pubmed/33299811
http://dx.doi.org/10.1016/j.btre.2020.e00566
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